Proteomics

Dataset Information

0

K29-linked unanchored polyubiquitin chains disrupt ribosome biogenesis and direct ribosomal proteins to the Intranuclear Quality control compartment (INQ)


ABSTRACT: Ribosome assembly requires precise coordination between the production and assembly of ribosomal components. Mutations in ribosomal proteins that inhibit the assembly process orribosome function are often associated with Ribosomopathies, some of which are linked to defects in proteostasis. In this study, we examine the interplay between several yeast proteostasis enzymes, including deubiquitylases (DUBs), Ubp2 and Ubp14, and E3 ligases, Ufd4 and Hul5, and we explore their roles in the regulation of the cellular levels of K29-linked unanchored polyubiquitin (polyUb) chains. Accumulating K29-linked unanchored polyUb chains associate with maturing ribosomes to disrupt their assembly, activate the Ribosome assembly stress response (RASTR), and lead to the sequestration of ribosomal proteins at the Intranuclear Quality control compartment INQ). These findings reveal the physiological relevance of INQ and provide insights into mechanisms of cellular toxicity associated with Ribosomopathies.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Eric Bonneil  

LAB HEAD: Brenda Andrews

PROVIDER: PXD042259 | Pride | 2023-08-10

REPOSITORIES: Pride

Dataset's files

Source:
altmetric image

Publications

K29-linked unanchored polyubiquitin chains disrupt ribosome biogenesis and direct ribosomal proteins to the Intranuclear Quality control compartment (INQ).

Suresh Harsha Garadi HG   Bonneil Eric E   Albert Benjamin B   Dominique Carine C   Costanzo Michael M   Pons Carles C   David Masinas Myra Paz MP   Shuteriqi Ermira E   Shore David D   Henras Anthony K AK   Thibault Pierre P   Boone Charles C   Andrews Brenda J BJ  

bioRxiv : the preprint server for biology 20230504


Ribosome assembly requires precise coordination between the production and assembly of ribosomal components. Mutations in ribosomal proteins that inhibit the assembly process or ribosome function are often associated with Ribosomopathies, some of which are linked to defects in proteostasis. In this study, we examine the interplay between several yeast proteostasis enzymes, including deubiquitylases (DUBs), Ubp2 and Ubp14, and E3 ligases, Ufd4 and Hul5, and we explore their roles in the regulatio  ...[more]

Similar Datasets

2023-05-16 | GSE232351 | GEO
2021-07-11 | PXD024425 | Pride
2024-09-30 | PXD047905 | Pride
2020-04-06 | PXD016166 | Pride
2014-11-14 | E-GEOD-52878 | biostudies-arrayexpress
2014-11-14 | GSE52878 | GEO
2021-01-14 | PXD023021 | Pride
2021-06-23 | PXD022703 | Pride
2024-06-22 | PXD046500 | Pride
2020-09-14 | GSE137951 | GEO