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Direct mass spectrometry-based detection and antibody sequencing of Monoclonal Gammopathy of Undetermined Significance from patient serum

ABSTRACT: Monoclonal gammopathy of undetermined significance (MGUS) is a plasma cell disorder, leading to the presence of monoclonal antibody (i.e., M-protein) in serum, without clinical symptoms. Here we present a case study in which we detect MGUS by liquid-chromatography coupled with mass spectrometry (LC-MS) profiling of IgG1 in human serum. We detected a Fab-glycosylated M-protein and determined the full heavy and light chain sequences by bottom-up proteomics techniques using multiple proteases, further validated by top-down LC-MS. Moreover, the composition and location of the Fab-glycan could be determined in CDR1 of the heavy chain.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Joost Snijder

LAB HEAD: Joost Snijder

PROVIDER: PXD042266 | Pride | 2023-09-14

REPOSITORIES: Pride

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			Action	DRS
	20210728_L1_Vq1_Tamar002_MGUS_Fab_TCEP_ETHCD_22min_rep1_01.raw	Raw
	20210728_L1_Vq1_Tamar002_MGUS_Fab_TCEP_FMS_22min_01.raw	Raw
	20211019_F1_UM4_Peng0013_MGUS_PNGaseF_chymo_02.raw	Raw
	20211019_F1_UM4_Peng0013_MGUS_PNGaseF_chymo_03.raw	Raw
	20220708_F1_UM4_Peng0013_MGUS_TL_01.raw	Raw

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