Dataset Information
Proteomic changes induced by knockdown of UBA1 and of E2 ubiquitin-conjugating enzymes in human HEK293 cells (UBE2Q1, UBE2QL1, UBER, UBE2S).Proteomic changes induced by knockdown of UBA1 and of E2 ubiquitin-conjugating enzymes in human HEK293 cells (Ctrl, CtrlHS, E2poolRNAi, E2poolRNAiHS)
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: 
Vishwajeeth Pagala
LAB HEAD: Fabio Demontis
PROVIDER: PXD042341 | Pride | 2024-01-26
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Demongrp_101321_hTMT_F1.1.pepXML | Pepxml | |||
| Demongrp_101321_hTMT_F1.raw | Raw | |||
| Demongrp_101321_hTMT_F10.1.pepXML | Pepxml | |||
| Demongrp_101321_hTMT_F10.raw | Raw | |||
| Demongrp_101321_hTMT_F11.1.pepXML | Pepxml |
Items per page: 1 - 5 of 60 |
Publications
An adaptive stress response that confers cellular resilience to decreased ubiquitination.
Nature communications 20231114 1
Ubiquitination is a post-translational modification initiated by the E1 enzyme UBA1, which transfers ubiquitin to ~35 E2 ubiquitin-conjugating enzymes. While UBA1 loss is cell lethal, it remains unknown how partial reduction in UBA1 activity is endured. Here, we utilize deep-coverage mass spectrometry to define the E1-E2 interactome and to determine the proteins that are modulated by knockdown of UBA1 and of each E2 in human cells. These analyses define the UBA1/E2-sensitive proteome and the E2 ...[more]
