Dataset Information

Shotgun Proteomics to characterize wastewater proteins

ABSTRACT: Wastewater has been extensively studied along the years. However, these studies have been focused on the analysis of small molecules. There are no studies about the proteins present in wastewater and let alone an established method to study them. We propose a method for the study of the proteins in wastewater overcoming their low concentration and the interference of other molecules. Moreover, we differentiate between the proteins that are soluble and the ones in the particulate. This method is based on concentration, lysis and clean-up steps. The samples were analyzed afterward using liquid chromatography coupled to high-resolution mass spectrometry (HR-LC/MS) and the data searched with Proteome Discoverer. Thus, this complete method has allowed us to characterize the proteomic composition of different wastewater samples with a low volume.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human) Bos Taurus (bovine) Gallus Gallus (chicken) Sus Scrofa Domesticus (domestic Pig) Mus Musculus (mouse)

SUBMITTER: Montserrat Carrascal

LAB HEAD: Montserrat Carrascal

PROVIDER: PXD042445 | Pride | 2023-10-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Re8467_MCP_esE13_Besos_Soluble_Influent_01_13-1_20230215.raw	Raw
	Re8467_MCP_esE13_Girona_Soluble_Influent_01_13-2_20230215.raw	Raw
	Re8467_MCP_esE13_Vic_Soluble_Influent_01_13-3_20230215.raw	Raw
	Re8467_MCP_esE28_Besos_Particulate_Influent_01_28-1_20230209.raw	Raw
	Re8467_MCP_esE28_Girona_Particulate_Influent_01_28-4_20230209.raw	Raw

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Shotgun proteomics to characterize wastewater proteins.

Sánchez-Jiménez Ester E Abian Joaquin J Ginebreda Antoni A Barceló Damià D Carrascal Montserrat M

MethodsX 20230926

Classically, the characterization of wastewater components has been restricted to the measurement of indirect parameters (chemical and biological oxygen demand, total nitrogen) and small molecules of interest in epidemiology or for environmental control. Despite the fact that metaproteomics has provided important knowledge about the microbial communities in these waters, practically nothing is known about other non-microbial proteins transported in the wastewater. The method described here has a ...[more]

PMID: 37854711

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