Proteomics

Dataset Information

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Cyclophilin A isomerization of Septin 2 mediates abscission during cytokinesis


ABSTRACT: The isomerase activity of Cyclophilin A is important for midbody abscission during cell division, however to date, midbody substrates remain unknown. In this study, we report that the GTP-binding protein Septin 2 interacts with Cyclophilin A. We highlight a dynamic series of Septin 2 phenotypes at the midbody, previously undescribed in human cells. Furthermore, Cyclophilin A depletion or loss of isomerase activity is sufficient to induce phenotypic Septin 2 defects at the midbody. Structural and molecular analysis reveals that Septin 2 proline 259 is important for interaction with Cyclophilin A. Moreover, an isomerisation-deficient EGFP-Septin 2 proline 259 mutant displays defective midbody localisation, and undergoes impaired abscission, consistent with data from cells with loss of Cyclophilin A expression or activity. Collectively, this data reveals Septin 2 as a novel interacting partner and isomerase substrate of Cyclophilin A at the midbody that is required for abscission during cytokinesis in cancer cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lymphoblastic Leukemia Cell Line, Lymphoblast

DISEASE(S): Myeloid Leukemia

SUBMITTER: Philip Cotter  

LAB HEAD: Margaret M. McGee

PROVIDER: PXD042822 | Pride | 2023-10-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20_01_14_DG1.raw Raw
20_01_14_DG10_140122165922.raw Raw
20_01_14_DG10_140122202154.raw Raw
20_01_14_DG11.raw Raw
20_01_14_DG11_140122212923.raw Raw
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Publications

Cyclophilin A Isomerisation of Septin 2 Mediates Abscission during Cytokinesis.

Gorry Rebecca L RL   Brennan Kieran K   Lavin Paul T M PTM   Mazurski Tayler T   Mary Charline C   Matallanas David D   Guichou Jean-François JF   Mc Gee Margaret M MM  

International journal of molecular sciences 20230704 13


The isomerase activity of Cyclophilin A is important for midbody abscission during cell division, however, to date, midbody substrates remain unknown. In this study, we report that the GTP-binding protein Septin 2 interacts with Cyclophilin A. We highlight a dynamic series of Septin 2 phenotypes at the midbody, previously undescribed in human cells. Furthermore, Cyclophilin A depletion or loss of isomerase activity is sufficient to induce phenotypic Septin 2 defects at the midbody. Structural an  ...[more]

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