Dataset Information

Quantitative proteomic analysis of MCM3 in ThinPrep samples of patients with cervical preinvasive cancer

ABSTRACT: Triage methods for cervical cancer detection show moderate accuracy and present considerable false-negative and false-positive result rates. A complementary diagnostic parameter could help improve the accuracy of identifying patients who need treatment. A pilot study was performed using a targeted proteomics approach with opportunistic ThinPrep samples obtained from women collected at the hospital’s outpatient clinic to determine the concentration levels of minichromosome maintenance-3 (MCM3) and envoplakin (EVPL) proteins. Forty samples with ’negative for intraepithelial lesion or malignancy’ (NILM), 21 samples with ’atypical squamous cells of undetermined significance’ (ASC-US), and 33 samples with ’low-grade squamous intraepithelial lesion and worse’ (≥LSIL) were analyzed, using cytology and the patients’ histology reports. Highly accurate concordance was obtained for gold standard-confirmed samples, demonstrating that the MCM3/EVPL ratio can discriminate between non-dysplastic and dysplastic samples. On that account, we propose that MCM3 and EVPL are promising candidate protein biomarkers for population-based cervical cancer screening.

INSTRUMENT(S): Orbitrap Fusion Lumos, Orbitrap Exploris 120

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endocervical Epithelium, Ectocervical Epithelium

DISEASE(S): Cervical Cancer

SUBMITTER: Coskun Guzel

LAB HEAD: Theo Luider

PROVIDER: PXD042918 | Pride | 2023-10-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2023.05.08_ThinPrep_patient_chart.xlsx	Xlsx
	221117OLc1_CG-2277-12-22-012-ThPrep-spSILs-und--PRM30_026.raw	Raw
	221117OLc1_CG-2277-13-22-013-ThPrep-spSILs-und--PRM30_027.raw	Raw
	221117OLc1_CG-2277-14-22-014-ThPrep-spSILs-und--PRM30_028.raw	Raw
	221117OLc1_CG-2277-15-22-015-ThPrep-spSILs-und--PRM30_029.raw	Raw

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Publications

Quantitative Proteomic Analysis of MCM3 in ThinPrep Samples of Patients with Cervical Preinvasive Cancer.

Köse Büşra B Laar Ralf van de RV Beekhuizen Heleen van HV Kemenade Folkert van FV Baykal Ahmet Tarik AT Luider Theo T Güzel Coşkun C

International journal of molecular sciences 20230621 13

Triage methods for cervical cancer detection show moderate accuracy and present considerable false-negative and false-positive result rates. A complementary diagnostic parameter could help improve the accuracy of identifying patients who need treatment. A pilot study was performed using a targeted proteomics approach with opportunistic ThinPrep samples obtained from women collected at the hospital's outpatient clinic to determine the concentration levels of minichromosome maintenance-3 (MCM3) an ...[more]

PMID: 37445651

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