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Deubiquitination of CDC6 by OTUD6A promotes tumour progression and chemoresistance

ABSTRACT: CDC6 is an oncogenic protein whose expression level fluctuates during the cell cycle. Although several E3 ubiquitin ligases responsible for the ubiquitin-mediated proteolysis of CDC6 have been identified, the deubiquitination pathway for CDC6 has not been investigated. Here, we report that OTUD6A functions as a deubiquitinase of CDC6. OTUD6A promotes cell proliferation and decreases sensitivity to chemotherapy by upregulating CDC6 protein expression.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: xc liu

LAB HEAD: Jianfeng Cui

PROVIDER: PXD042935 | Pride | 2024-05-24

REPOSITORIES: Pride

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			Action	DRS
	LD206LQ_Flag_Slot1-4_1_5652.d.zip	Other
	LD206LQ_OTUD6A_Slot1-5_1_5654.d.zip	Other
	checksum.txt	Txt
	txt.zip	Other

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Deubiquitination of CDC6 by OTUD6A promotes tumour progression and chemoresistance.

Cui Jianfeng J Liu Xiaochen X Shang Qinghong Q Sun Shuna S Chen Shouzhen S Dong Jianping J Zhu Yaofeng Y Liu Lei L Xia Yangyang Y Wang Yong Y Xiang Lu L Fan Bowen B Zhan Jiafeng J Zhou Yadi Y Chen Pengxiang P Zhao Renchang R Liu Xiaofei X Xing Nianzeng N Wu Dalei D Shi Benkang B Zou Yongxin Y

Molecular cancer 20240429 1

<h4>Background</h4>CDC6 is an oncogenic protein whose expression level fluctuates during the cell cycle. Although several E3 ubiquitin ligases responsible for the ubiquitin-mediated proteolysis of CDC6 have been identified, the deubiquitination pathway for CDC6 has not been investigated.<h4>Methods</h4>The proteome-wide deubiquitinase (DUB) screening was used to identify the potential regulator of CDC6. Immunofluorescence, protein half-life and deubiquitination assays were performed to determine ...[more]

PMID: 38685067

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