Proteomic analysis of extracellular vesicle released by MCF7 cells in the absence of the tetraspanins CD9, CD81 or CD63
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ABSTRACT: The tetraspanins CD9, CD81, and CD63 are among the most widely used markers of Extracellular Vesicles (EVs) and are also used to isolate the vesicles they decorate. If their function in EVs is largely unknown, they have been suggested to play a role in the sorting of protein cargos into EVs or to regulate the uptake of EVs by acceptor cells. Using different tetraspanin-deficient MCF7 breast cancer cell lines, we have shown that the absence of any of these 3 tetraspanins had a minimal impact on their protein composition, as analyzed by label-free quantitative mass spectrometry. However, the EVs released by cells deficient in both CD9 and CD81 showed a decrease level of CD9P-1/EWI-F and EWI-2 (encoded by the genes PTGFRN and IGSF8 respectively), which are two well-characterized CD9 and CD81 molecular partners.
INSTRUMENT(S): timsTOF Pro
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Extracellular Vesicle, Breast Cancer Cell Line, Cell Line Cell
SUBMITTER: Cédric Pionneau
LAB HEAD: Eric RUBINSTEIN
PROVIDER: PXD042940 | Pride | 2023-08-03
REPOSITORIES: Pride
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