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Nardilysin-Regulated Scission Mechanism Activates Polo-like Kinase 3 to Suppress the Development of Pancreatic Cancer

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) develops through step-wise genetic and molecular alterations including Kras mutation and inactivation of apoptotic pathways. Here, we find that development of anoikis resistance and metastasis of KrasG12D-driven PDAC in mice is accelerated by deleting Plk3, explaining the often reduced Plk3 expression in human PDAC. Importantly, a 41 kDa Plk3 (p41Plk3) that contained the entire kinase domain at the N-terminus (1-353 aa) is activated by scission of the precursor p72Plk3 at Arg354 by metalloendopeptidase Nardilysin (NRDC), and the resulting p32Plk3 C-terminal Polo-box domain (PBD) was quickly removed by proteasome degradation preventing the p41Plk3 inhibition by PBD. We found that p41Plk3 is the activated form of Plk3 that regulates a feedforward mechanism to promote anoikis and suppress PDAC and metastasis. p41Plk3 phosphorylates c-Fos on Thr164, which in turn, induces expression of Plk3 and pro-apoptotic genes. These findings uncovered an NRDC-regulated post-translational mechanism (PTM) that activates Plk3, establishing a prototypic regulation by scission mechanism.

INSTRUMENT(S): Voyager-DE PRO

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Paul Chiao

LAB HEAD: Paul J Chiao

PROVIDER: PXD042993 | Pride | 2023-12-23

REPOSITORIES: Pride

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			Action	DRS
	Plk1_Mu_incubated-Supplementary-Figure4.raw	Raw
	Plk1_WT_incubated-Supplementary-Figure4.raw	Raw
	Plk3_Mu_incubated-Supplementary-Figure3h.raw	Raw
	Plk3_WT_incubated-Supplementary-Figure3h.raw	Raw
	Plk3_cFos-lowerHCD-Fig6-and-Supplementary-Fig7a.raw	Raw

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