Proteomics

Dataset Information

0

A protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus Magnaporthe oryzae within rice cells


ABSTRACT: The devastating blast fungus Magnaporthe oryzae elaborates invasive hyphae (IH) in living rice cells during early infection, separated from host cytoplasm by plant-derived interfacial membranes, but the metabolic strategies underpinning this fundamental intracellular biotrophic growth phase are poorly understood. Eukaryotic cell growth depends on activated target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Here, using live-cell imaging coupled with multiomic approaches, we show how the M. oryzae serine/threonine protein kinase Rim15 coordinates cycles of autophagy and glutaminolysis in IH – the latter through phosphorylation of NAD-dependent glutamate dehydrogenase – to reactivate TOR and promote biotrophic growth. Deleting RIM15 attenuated IH growth and triggered plant immunity; these defects were fully remediated by exogenous α-ketoglutarate treatment, but glucose treatment only suppressed host defenses. Our results together suggest that Rim15-dependent cycles of autophagic flux liberate α-ketoglutarate – via glutaminolysis – to reactivate TOR signaling and fuel biotrophic growth while conserving glucose for antioxidation-mediated host innate immunity suppression.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Magnaporthe Sp. Mg08

TISSUE(S): Mycelial Felt, Liver

SUBMITTER: Gang Li  

LAB HEAD: Richard A. Wilson

PROVIDER: PXD043076 | Pride | 2023-07-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20210522_GL_Guy11-R1_1ug.raw Raw
20210522_GL_Guy11-R2_1ug.raw Raw
20210522_GL_Guy11-R3_1ug.raw Raw
20210522_GL_Mascot-1.msf Msf
20210522_GL_rim15-R1_1ug.raw Raw
Items per page:
1 - 5 of 15

Similar Datasets

2009-10-02 | GSE18361 | GEO
2009-10-09 | E-GEOD-18361 | biostudies-arrayexpress
2011-01-24 | E-GEOD-21908 | biostudies-arrayexpress
2009-04-08 | E-GEOD-8670 | biostudies-arrayexpress
2016-04-01 | E-GEOD-73096 | biostudies-arrayexpress
2013-04-05 | E-GEOD-40245 | biostudies-arrayexpress
2012-04-29 | E-GEOD-33542 | biostudies-arrayexpress
2010-09-17 | E-GEOD-18193 | biostudies-arrayexpress
| PRJNA97177 | ENA
2021-01-17 | E-MTAB-9984 | biostudies-arrayexpress