Dataset Information

An integrative proteomics approach to identify novel therapeutic targets for SPINK1-positive prostate cancer.

ABSTRACT: Dysregulated phosphorylation of proteins perturbs the activity of several biological pathways leading to tumorigenesis and metastases. Integrated proteomics studies offer an in-depth comprehension of cancer pathobiology, and could untangle its complex circuitries. To decipher the signaling involved in SPINK1-mediated tumorigenesis, we performed label-free quantitative proteome and phosphoproteome profiling of small hairpin RNA -mediated SPINK1 silenced (shSPINK1) and control small hairpin RNA Scrambled (shSCRM) 22RV1 cells. Our integrated proteomics data revealed ~4000 proteins and enriched ~6000 phosphopeptides in each biological replicate. Of these, 367 proteins and 807 phosphopeptides were found to be significantly altered between shSCRM and shSPINK1 cells.

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Bushra Ateeq

LAB HEAD: Dr. Bushra Ateeq

PROVIDER: PXD043086 | Pride | 2024-02-26

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	22RV1_LFQ_Proteome_analysis.pdResult	Other
	22RV1_Phopshoproteome_analysis.pdResult	Other
	419_1_Phospho.raw	Raw
	419_1_Proteome.raw	Raw
	419_2_Phospho.raw	Raw

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Publications

An integrative proteomics approach identifies tyrosine kinase KIT as a therapeutic target for SPINK1-positive prostate cancer.

Manzar Nishat N Khan Umar Khalid UK Goel Ayush A Carskadon Shannon S Gupta Nilesh N Palanisamy Nallasivam N Ateeq Bushra B

iScience 20240104 3

Elevated serine peptidase inhibitor, Kazal type 1 (SPINK1) levels in ∼10%-25% of prostate cancer (PCa) patients associate with aggressive phenotype, for which there are limited treatment choices and dismal clinical outcomes. Using an integrative proteomics approach involving label-free phosphoproteome and proteome profiling, we delineated the downstream signaling pathways involved in SPINK1-mediated tumorigenesis and identified tyrosine kinase KIT as highly enriched. Furthermore, high to moderat ...[more]

PMID: 38384854

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