Dataset Information

ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression

ABSTRACT: CIITA is the master regulator of MHC II genes expression and hence the adaptive immune response. CIITA expression itself is tightly regulated by three cell type-specific promoters, pI, pIII, and by pIV, and can also be induced by IFNg in non-immune cells. While key regulatory elements have been identified within these promoters, knowledge of transcription factors regulating CIITA is incomplete. Here, we demonstrate that the telomere-binding protein and transcriptional activator ZBTB48 directly binds to both critical activating elements within CIITA pIII and is essential for its gene expression. ZBTB48 establishes open chromatin at CIITA pIII upstream of activating H3K4me3 modifications both priming CIITA transcription for IFNg-induction and ensuring constitutive expression in primary murine B cells. Hence, ZBTB48 acts as a molecular on-off-switch for B-cell-specific CIITA expression.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Osteosarcoma

SUBMITTER: Dennis Kappei

LAB HEAD: Dennis Kappei

PROVIDER: PXD043134 | Pride | 2024-12-22

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20191015_LAB_CC_U2OS_WT_IFNg_01_a.raw	Raw
	20191015_LAB_CC_U2OS_WT_IFNg_01_b.raw	Raw
	20191015_LAB_CC_U2OS_WT_IFNg_01_c.raw	Raw
	20191015_LAB_CC_U2OS_WT_IFNg_01_d.raw	Raw
	20191016_LAB_CC_U2OS_KO_IFNg_C3_a.raw	Raw

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ZBTB48 is a priming factor regulating B-cell-specific CIITA expression.

Rane Grishma G Kuan Vivian L S VLS Wang Suman S Mok Michelle Meng Huang MMH Khanchandani Vartika V Hansen Julia J Norvaisaite Ieva I Zulkaflee Naasyidah N Yong Wai Khang WK Jahn Arne A Mukundan Vineeth T VT Shi Yunyu Y Osato Motomi M Li Fudong F Kappei Dennis D

The EMBO journal 20241119 24

The class-II transactivator (CIITA) is the master regulator of MHC class-II gene expression and hence the adaptive immune response. Three cell type-specific promoters (pI, pIII, and pIV) are involved in the regulation of CIITA expression, which can be induced by IFN-γ in non-immune cells. While key regulatory elements have been identified within these promoters, our understanding of the transcription factors regulating CIITA expression is incomplete. Here, we demonstrate that the telomere-bindin ...[more]

PMID: 39562739

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