Dataset Information

Proteomic profiling for elucidating the molecular mechanism in early seed development in rice

ABSTRACT: Understanding the molecular mechanisms underlying early seed development is important for improving grain yield and quality in crop plants. To investigate the molecular mechanisms that occur during early seed development, we performed comparative label-free quantitative proteomic analysis on developing WT rice seeds between 0 and 1 day after pollination (DAP). A total of 5231 proteins were identified, and 902 proteins showed differential accumulation between 0 and 1 DAP seeds. Further analysis focused on the proteins preferentially expressed at 1 DAP and revealed an enrichment of proteins involved in DNA replication and pyrimidine biosynthetic pathways. Notably, the transcript of OsCTPS1, a cytidine triphosphate synthase known to play an essential role in early endosperm development, did not differ between 0 and 1 DAP, but OsCTPS1 protein accumulated more specifically at 1 DAP than at 0 DAP. We then found that inhibiting phosphorylation increased the stability of this protein. Furthermore, in osctps1-2, minichromosome maintenance (MCM) proteins were significantly reduced compared to WT at 1 DAP, and mutations in OsMCM5 caused defects in seed development. In this study, we tried to understand the molecular mechanisms of early seed development at the post-transcriptional regulation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Ovary

SUBMITTER: Sun Tae Kim

LAB HEAD: Sun Tae Kim

PROVIDER: PXD043162 | Pride | 2023-12-05

REPOSITORIES: Pride

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Publications

Quantitative Proteomic Analysis Deciphers the Molecular Mechanism for Endosperm Nuclear Division in Early Rice Seed Development.

Yoon Jinmi J Min Cheol Woo CW Kim Jiyoung J Baek Gibeom G Kim Dohyeon D Jang Jeong Woo JW Gupta Ravi R Kim Sun Tae ST Cho Lae-Hyeon LH

Plants (Basel, Switzerland) 20231029 21

Understanding the molecular mechanisms underlying early seed development is important in improving the grain yield and quality of crop plants. We performed a comparative label-free quantitative proteomic analysis of developing rice seeds for the WT and osctps1-2 mutant, encoding a cytidine triphosphate synthase previously reported as the endospermless 2 (enl2) mutant in rice, harvested at 0 and 1 d after pollination (DAP) to understand the molecular mechanism of early seed d ...[more]

PMID: 37960070

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Project description:Plants initially undergo a period of vegetative development, in which it produces leaves from shoot apical meristem (SAM) and roots from the root apical meristem. Later in development, the SAM undergoes a change in fate and enters reproductive development called as floral transition, producing flowers and seeds. Our understanding of the molecular and genetic mechanisms that underlie reproductive development in plants has increased tremendously in the past decade, essentially through the work on Arabidopsis. In this study, we have analyzed the spatial and temporal gene expression in various tissues/organs and developmental stages of rice using microarray technology to identify the genes differentially expressed during various stages of reproductive development. Experiment Overall Design: Various rice tissues/organs (seedling root, mature leaf and Y leaf) and stages of reproductive (panicle and seed) development were used for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates of each sample were used for microarray analysis. We sought to obtain developmental and temporal gene expression profiles at each stage to identify the differentially expressed genes. Different stages of panicle and seed development have been categorized according to panicle length and days after pollination, respectively, based on landmark developmental events as follows: up to 0.5 mm, shoot apical meristem and rachis meristem (SAM); 0-3 cm, floral transition and floral organ development (P1); 3-10 cm, meiotic stage (P2 and P3); 10-15 cm, young microspore stage (P4); 15-22 cm, vacuolated pollen stage (P5); 22-30 cm, mature pollen stage (P6); 0-2 dap, early globular embryo (S1); 3-4 dap, middle and late globular embryo (S2); 5-10 dap, embryo morphogenesis (S3); 11-20 dap, embryo maturation (S4); 21-29, dormancy and desiccation tolerance (S5). These stage specifications are approximations based on information from Itoh et al., 2005, Plant Cell Physiol, 46, 23-47.

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