Proteomics

Dataset Information

0

Plasmodium ARK2 and EB1 drives the unconventional spindle dynamics, during chromosome segregation in sexual transmission stages

ABSTRACT: The Aurora family of kinases orchestrates chromosome segregation and cytokinesis during cell division, with precise spatiotemporal regulation of its catalytic activities by distinct protein scaffolds. Plasmodium spp., the causative agents of malaria, are unicellular eukaryotes with three unique and highly divergent aurora-related kinases (ARK1-3) that are essential for asexual cellular proliferation, but lack most canonical scaffolds/activators. Here we investigate the role of ARK2 during sexual proliferation of the rodent malaria Plasmodium berghei, using a combination of super-resolution microscopy, mass spectrometry, and live-cell fluorescence imaging. We find that ARK2 is primarily located at spindle apparatus microtubules in the vicinity of kinetochores during both mitosis and meiosis. Interactomic and co-localisation studies reveal several putative ARK2-associated interactors including the microtubule plus end-binding protein EB1, together with MISFIT and Myosin-K, but no conserved eukaryotic scaffold proteins. Gene function studies indicate that ARK2 and EB1 are complementary in driving endomitotic division and thereby parasite transmission through the mosquito. This discovery underlines the flexibility of molecular networks to rewire and drive unconventional mechanisms of chromosome segregation in the malaria parasite.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Plasmodium Berghei Anka

SUBMITTER: Andrew Bottrill  

LAB HEAD: Professor Rita Tewari

PROVIDER: PXD043164 | Pride | 2023-08-15

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Ark2-GFP_1.MGF Mgf
Ark2-GFP_1.mzid.gz Mzid
Ark2-GFP_1.raw Raw
Ark2-GFP_2.MGF Mgf
Ark2-GFP_2.mzid.gz Mzid
Items per page:
1 - 5 of 18
altmetric image

Publications

Mechanisms of cell division are remarkably diverse, suggesting the underlying molecular networks among eukaryotes differ extensively. The Aurora family of kinases orchestrates the process of chromosome segregation and cytokinesis during cell division through precise spatiotemporal regulation of their catalytic activities by distinct scaffolds. Plasmodium spp., the causative agents of malaria, are unicellular eukaryotes that have three divergent aurora-related kinases (ARKs) and lack most canonic  ...[more]

Similar Datasets

Proteomics Real-time dynamics of Plasmodium Ndc80 reveals unusual modes of chromosome segregation during parasite proliferative stages
2020-06-09 | PXD017619 | Pride
Proteomics Plasmodium NEK1 coordinates MTOC organisation and kinetochore attachment during rapid mitosis in male gamete formation
2024-08-08 | PXD053559 | Pride
Proteomics The Skp1-Cullin1-FBXO1 complex is a pleiotropic regulator required for the formation of gametes and zoites in Plasmodium berghei
2023-02-17 | PXD035526 | Pride
Proteomics Plasmodium condensin core subunits (SMC2/SMC4) mediate atypical mitosis and are essential for parasite proliferation and transmission
2020-05-27 | PXD016833 | Pride
Proteomics The Skp1-Cullin1-FBXO1 complex is a pleiotropic regulator required for the formation of gametes and zoites in Plasmodium berghei
2023-02-17 | PXD035557 | Pride
Transcriptomics Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes: molecular insight into sex-specific processes and translational repression
2016-06-28 | E-GEOD-75795 | biostudies-arrayexpress
Transcriptomics Malaria Cell Atlas: Single cell transcriptomics of malaria parasites
2017-02-08 | E-ERAD-611 | biostudies-arrayexpress
Transcriptomics Global expression profiling reveals shared and distinct transcript signatures in arrested act2(-) and CDPK4(-) Plasmodium berghei gametocytes
2015-09-15 | E-GEOD-65032 | biostudies-arrayexpress
Transcriptomics Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in the Plasmodium gametocyte
2014-10-23 | E-MTAB-2900 | biostudies-arrayexpress
Proteomics A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of Plasmodium berghei during gametogony and parasite transmission.
2020-06-17 | PXD017622 | Pride