Dataset Information

Mass spectrometric ITEM-ONE analysis confirms and refines an assembled affimer binding site on the HER-2 targeting therapeutic antibody Pertuzumab

ABSTRACT: The Pertuzumab-binding affimer 00557_709097 was incubated either with peptides from tryptically digested Pertuzumab or with selected synthesized peptides. Affimer-peptide complexes were subjected to nano electrospray mass spectrometric ITEM analyses in order to identify the affimer’s epitope on Pertuzumab. Furthermore the affimer was incubated with intact Pertuzmab to determine apparent binding energies and dissociation constants of the complex dissociation reactions in the gas phase using ITEM-TWO.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Michael Kreutzer

LAB HEAD: Prof. Dr. Michael O. Glocker

PROVIDER: PXD043203 | Pride | 2024-04-09

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	AFFIMER_HISPEP.raw.zip	Raw
	AFFIMER_PERTUPEP54-67.raw.zip	Raw
	AFFIMER_PERTUPEP54-74.raw.zip	Raw
	AFFIMER_PERTUPEP68-74.raw.zip	Raw
	AFFIMER_PERTUZUMAB.raw.zip	Raw

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Publications

Mass Spectrometric ITEM-ONE and ITEM-TWO Analyses Confirm and Refine an Assembled Epitope of an Anti-Pertuzumab Affimer.

Röwer Claudia C Olaleye Oladapo O OO Bischoff Rainer R Glocker Michael O MO

Biomolecules 20231224 1

Intact Transition Epitope Mapping-One-step Non-covalent force Exploitation (ITEM-ONE) analysis reveals an assembled epitope on the surface of Pertuzumab, which is recognized by the anti-Pertuzumab affimer 00557_709097. It encompasses amino acid residues NSGGSIYNQRFKGR, which are part of CDR2, as well as residues FTLSVDR, which are located on the variable region of Pertuzumab's heavy chain and together form a surface area of 1381.46 Å<sup>2</sup>. Despite not being part of Pertuzumab's CDR2, the ...[more]

PMID: 38254624

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