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Proteomic profiles of bona fide RNA-binding proteins across E. coli growth curve in M9LB media.

ABSTRACT: Dynamic interactions between RNA and RNA-binding proteins (RBPs) regulate a broad spectrum of bacterial functions. Here, we have characterised how the RBPome is dynamically rewired during the E. coli life cycle. We applied Orthogonal Organic Phase Separation (OOPS) coupled with an RNase digestion step to shortlist bona fide bacterial RBPs and assessed whether proteins bound RNA differentially express at different cell proliferation stages.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Escherichia Coli

SUBMITTER: Eneko Villanueva

LAB HEAD: Prof. Kathryn S Lilley

PROVIDER: PXD043373 | Pride | 2024-03-20

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Metadata PRIDE submission.xlsx	Xlsx
	MetadataPRIDEsubmission.xlsx	Xlsx
	Mie_B1.msf	Msf
	Mie_B1.raw	Raw
	Mie_B2.msf	Msf

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