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HEATR5B associates with dynein-dynactin and selectively promotes motility of AP1- bound endosomal membranes_BioMass@LMB

ABSTRACT: The dynein motor complex mediates polarised trafficking of a wide variety of organelles, intracellular vesicles and macromolecules. These functions are dependent on the dynactin 5 complex, which helps recruit cargoes to dynein’s tail region and activates motor movement. How dynein and dynactin orchestrate trafficking of diverse cargoes is unclear. Here, we identify HEATR5B, an interactor of the AP1 clathrin adaptor complex, as a novel player in dynein-dynactin function. HEATR5B is one of several proteins recovered in a biochemical screen for proteins whose association with the human dynein tail complex is augmented by 10 dynactin. We show that HEATR5B binds directly to the dynein tail and dynactin and stimulates motility of AP1-associated endosomal membranes in human cells. We also demonstrate that the HEATR5B homologue in Drosophila is an essential gene that promotes dynein-based transport of AP1-bound membranes to the Golgi apparatus. As HEATR5B lacks the coiledcoil architecture typical of dynein adaptors, our data point to a non-canonical process 15 orchestrating motor function on a specific cargo. We additionally show that HEATR5B promotes association of AP1 with endosomal membranes in a dynein-independent manner. Thus, HEATR5B co-ordinates multiple events in AP1-based trafficking.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Drosophila Melanogaster (fruit Fly) Mus Musculus (mouse)

TISSUE(S): Brain, Cell Culture

SUBMITTER: Catarina Franco

LAB HEAD: Simon L.

PROVIDER: PXD043387 | Pride | 2023-10-20

REPOSITORIES: pride

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			Action	DRS
	MCT3166A51LC3_A1.raw	Raw
	MCT3166A51MA11_F100021_A1.dat	Other
	MCT3166A52LC3_B1.raw	Raw
	MCT3166A52MA12_F100032_B1.dat	Other
	MCT3166A53LC3_C1.raw	Raw

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