Proteomics

Dataset Information

0

Streptococcus agalactiae and Prototheca spp. infections induce changes in milk peptidome in Holstein cattle


ABSTRACT: Early detection of bovine subclinical mastitis may improve treatment strategies and reduce the use of antibiotics. Herein, individual milk samples from Holstein cows affected by subclinical mastitis induced by S. agalactiae and Prototheca spp. were analyzed by untargeted and targeted mass spectrometry approaches to assess changes in their peptidome profiles and identify new potential biomarkers of the pathological condition. Results showed a higher amount of peptides in milk positive at the bacteriological examination when compared with the negative control. However, the different pathogens seemed not to trigger specific effects on milk peptidome. The peptides that best distinguish positive from negative samples are mainly derived from the most abundant milk proteins, especially from β- and αs1-casein, but also include the antimicrobial peptide casecidin 17. These results provide new insights into the physiopathology of mastitis. Upon further validation, the panel of potential discriminant peptides could help to the development of new diagnostic and therapeutic tools.

INSTRUMENT(S): LTQ Orbitrap, TSQ Quantiva

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Giorgio Arrigoni  

LAB HEAD: Giorgio Arrigoni

PROVIDER: PXD043426 | Pride | 2024-01-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
07112022_Latte_Agalactiae_15.raw Raw
07112022_Latte_Agalactiae_17.raw Raw
07112022_Latte_Negativo_4.raw Raw
07112022_Latte_Negativo_73.raw Raw
07112022_Latte_Negativo_77.raw Raw
Items per page:
1 - 5 of 73
altmetric image

Publications

Subclinical Mastitis from <i>Streptococcus agalactiae</i> and <i>Prototheca</i> spp. Induces Changes in Milk Peptidome in Holstein Cattle.

Vanzin Alice A   Franchin Cinzia C   Arrigoni Giorgio G   Battisti Ilaria I   Masi Antonio A   Squartini Andrea A   Bisutti Vittoria V   Giannuzzi Diana D   Gallo Luigi L   Cecchinato Alessio A   Pegolo Sara S  

Journal of agricultural and food chemistry 20231027 44


Early detection of bovine subclinical mastitis may improve treatment strategies and reduce the use of antibiotics. Herein, individual milk samples from Holstein cows affected by subclinical mastitis induced by <i>S. agalactiae</i> and <i>Prototheca</i> spp. were analyzed by untargeted and targeted mass spectrometry approaches to assess changes in their peptidome profiles and identify new potential biomarkers of the pathological condition. Results showed a higher amount of peptides in milk positi  ...[more]

Similar Datasets

2020-07-15 | PXD017768 | Pride
2021-05-26 | PXD022595 | Pride
2020-04-09 | PXD017243 | Pride
2019-10-22 | PXD012355 | Pride
2022-08-31 | GSE201588 | GEO
2022-06-09 | PXD028793 | Pride
2022-03-16 | GSE149856 | GEO
2020-08-19 | PXD016762 | Pride
2015-01-21 | E-GEOD-65152 | biostudies-arrayexpress
2016-04-01 | E-GEOD-77978 | biostudies-arrayexpress