Proteomics

Dataset Information

0

Therapeutic potential of pomegranate juice-derived nanovesicles in nude mouse benign prostatic hyperplasia (BPH) xenograft model


ABSTRACT: Benign prostatic hyperplasia (BPH) and associated lower urinary tract symptoms affect a large percentage of the male population and places a substantial burden on the world health system. Current therapies include 5-alpha reductase inhibitors and alpha-blockers that are only partially effective and pose a huge economic burden, emphasizing the urgent need for effective, economical therapies. We isolated nanovesicles from pomegranate juice (Punica Granatum) (referred to as ‘POM-NVs’) and report to our knowledge for the first time, that these vesicles possess therapeutic potential against BPH. Following extensive characterization of POM-NVs, we tested their therapeutic potential in vitro using BPH1 cell line and identified a potential anti-proliferative and pro-apoptotic effect. We further tested these vesicles using a clinically relevant xenograft mouse BPH model derived from human BPH tissues. Remarkably, POM-NVs could reverse the BPH phenotype conferred by TGF-β mediated signaling and induced epithelial-to-mesenchymal (EMT) reversal, leading to the restoration of prostate epithelial states in vivo and in vitro. Furthermore, these vesicles attenuated bone morphogenic protein 5 (BMP5) signaling, a cardinal alteration that is instrumental in driving BPH. Considering the large incidences of BPH and its associated economic burdens, our study has important implications and can potentially improve the clinical management of BPH.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): 'punica Granatum' Phytoplasma Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Sharanjot Saini  

LAB HEAD: Sharanjot Saini

PROVIDER: PXD043608 | Pride | 2023-07-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AS1.msf Msf
AS1.raw Raw
AS2.msf Msf
AS2.raw Raw
POM_exosome.msf Msf
Items per page:
1 - 5 of 7

Similar Datasets

2022-07-13 | GSE183220 | GEO
2021-07-07 | GSE146500 | GEO
2019-11-27 | GSE136627 | GEO
2014-09-01 | E-GEOD-56825 | biostudies-arrayexpress
2022-01-05 | PXD010539 | Pride
2024-08-31 | E-MTAB-14168 | biostudies-arrayexpress
2023-07-22 | PXD043983 | Pride
2023-10-24 | PXD044065 | Pride
2014-11-24 | E-GEOD-57713 | biostudies-arrayexpress
2022-12-12 | PXD036431 | Pride