Proteomics

Dataset Information

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Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search

ABSTRACT: We evaluated the performance of the Orbitrap Ascend in comparison to the Orbitrap Eclipse in RTS-SPS-MS3-based analysis of human proteomes using fractionated and unfractionated samples labeled with TMT and TMTpro reagents. We did this comparison in the context of different sample input amounts; we analyzed differences in quantitative precision; we compared the effectiveness in identifying protein abundance changes induced by treatment with covalent inhibitors of PIN1.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Steven Shuken  

LAB HEAD: Qing Yu

PROVIDER: PXD043660 | Pride | 2023-10-13

REPOSITORIES: Pride

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Dataset's files

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20220722_Eclipse_2ProteomeMix_Keese_qysrsColumn_HYpro_TMT16_G65min_HrMS2_HCD36_IT54_30K_phiSDM_028.raw Raw
20220722_Eclipse_2ProteomeMix_Keese_qysrsColumn_HYpro_TMT16_G65min_HrMS2_HCD36_IT86_027.raw Raw
20220722_Eclipse_2ProteomeMix_Keese_qysrsColumn_HYpro_TMT16_G65min_RTSMS3_CID34IT23MS2_HCD55MS3IT105ms_029.raw Raw
20220722_Eclipse_2ProteomeMix_Keese_qysrsColumn_HYpro_TMT16_G65min_RTSMS3_CID34IT23MS2_HCD55MS3IT54ms_30K_phiSDM_030.raw Raw
20220726_Ascend_2ProteomeMix_Keese_qysrsColumn_HYpro_TMT16_G65min_HrMS2_HCD36_IT54_30K_phiSDM_028.raw Raw
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Publications

Deep Proteomic Compound Profiling with the Orbitrap Ascend Tribrid Mass Spectrometer Using Tandem Mass Tags and Real-Time Search.

Shuken Steven R SR   McAlister Graeme C GC   Barshop William D WD   Canterbury Jesse D JD   Bergen David D   Huang Jingjing J   Huguet Romain R   Paulo João A JA   Zabrouskov Vlad V   Gygi Steven P SP   Yu Qing Q  

Analytical chemistry 20231009 41

Tandem mass tags (TMT) and tribrid mass spectrometers are a powerful combination for high-throughput proteomics with high quantitative accuracy. Increasingly, this technology is being used to map the effects of drugs on the proteome. However, the depth of proteomic profiling is still limited by sensitivity and speed. The new Orbitrap Ascend mass spectrometer was designed to address these limitations with a combination of hardware and software improvements. We evaluated the performance of the Asc  ...[more]

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