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Alanine Scanning-PRISMA of GATAD1 peptide

ABSTRACT: To explore the role of individual amino acids in the binding of GATAD1 peptide (position 95-109) to 14-3-3 proteins, we conducted an alanine scanning experiment. Each amino acid surrounding the phosphorylation site within the peptide was systematically mutated to alanine, except for the original alanine residues, which were mutated to glycine. These mutant peptides were immobilized on a cellulose membrane and employed to capture proteins from Hek293T lysate through pulldown assays.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Trendelina Rrustemi

LAB HEAD: Matthias Selbach

PROVIDER: PXD043788 | Pride | 2024-02-15

REPOSITORIES: Pride

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			Action	DRS
	Rowlf_20221020_TR_HS_AlaScanning_1_1.raw	Raw
	Rowlf_20221020_TR_HS_AlaScanning_1_10.raw	Raw
	Rowlf_20221020_TR_HS_AlaScanning_1_11.raw	Raw
	Rowlf_20221020_TR_HS_AlaScanning_1_12.raw	Raw
	Rowlf_20221020_TR_HS_AlaScanning_1_13.raw	Raw

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