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Defining neutralization and allostery by antibodies against COVID-19 variants

ABSTRACT: This project is aimed at characterizing the interactions of SARS-CoV-2 Spike protein and its variants with multiple full-length antibodies and monitoring the accompanying conformational dynamics. Different categories of antibodies are tested that recognize different domains of the Spike protein. The project aims at identifying the effects of weak, moderate and strong neutralizing antibodies on Spike protein and decipher their mechanisms of action. In addition to the direct binding effects, distal allosteric effects are also determined. A range of biophysical experiments, biochemical assays, and molecular dynamics simulations are used as orthogonal approaches. The rationale is to identify regions on the SARS-CoV-2 Spike protein that acts as indicators for antibody binding and use these hotspots to develop better neutralizing antibodies against SARS-CoV-2 and any future viral pandemics.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Nikhil Kumar Tulsian

LAB HEAD: Paul MacAry

PROVIDER: PXD043818 | Pride | 2023-10-09

REPOSITORIES: Pride

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			Action	DRS
	02Aug21_Spike_UnD1_20210802181752.zip	Other
	02Aug21_Spike_UnD2_20210802183809.zip	Other
	02Mar22_NKT_RBD_O2_UnD1_20220302162358.zip	Other
	02Mar22_NKT_RBD_O2_UnD2_20220302235021.zip	Other
	02Mar22_NKT_RBD_O2_UnD3_20220303002435.zip	Other

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