The IP-MS results of anti-strep and protein A/G in VdΔulpb/Strep-SUMO strain
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ABSTRACT: To identify SUMO-modified proteins and determine the potential SUMO-specific protease target, we first transformed a Strep-VdSUMO construct into VdΔulpb to create the VdΔulpb/Strep-SUMO strain for immunoprecipitation with anti-Strep and protein A/G magnetic beads as negtive control. An anti-Strep-IPed sample was used for mass spectrometry (MS), and twenty-one potential proteins were obtained.
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Verticillium Dahliae Vdls.17
TISSUE(S): Fungus
SUBMITTER: bosen zhang
LAB HEAD: huishan guo
PROVIDER: PXD043859 | Pride | 2023-08-01
REPOSITORIES: Pride
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