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The IP-MS results of anti-strep and protein A/G in VdΔulpb/Strep-SUMO strain

ABSTRACT: To identify SUMO-modified proteins and determine the potential SUMO-specific protease target, we first transformed a Strep-VdSUMO construct into VdΔulpb to create the VdΔulpb/Strep-SUMO strain for immunoprecipitation with anti-Strep and protein A/G magnetic beads as negtive control. An anti-Strep-IPed sample was used for mass spectrometry (MS), and twenty-one potential proteins were obtained.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Verticillium Dahliae Vdls.17

TISSUE(S): Fungus

SUBMITTER: bosen zhang

LAB HEAD: huishan guo

PROVIDER: PXD043859 | Pride | 2023-08-01

REPOSITORIES: pride

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			Action	DRS
	Peptide-F002593.csv	Csv
	R20191201709_S1_4.raw	Raw
	Strep_NC.raw	Raw
	proteins.xlsx	Xlsx

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IPed-MS results with anti-Strep in VdΔulpb/Strep-SUMO strain.

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