Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Improved the Anti-MRSA Activity of Brevibacillus sp. SPR20

ABSTRACT: Brevibacillus sp. SPR20 produced potentially antibacterial substances against MRSA. The synthe-sis of these substances is controlled by their biosynthetic gene clusters. Several mutagenesis methods are used to overcome the restriction of gene regulations when genetic information is ab-sent. Atmospheric and room temperature plasma (ARTP) is a powerful technique to make random mutagenesis for microbial strain improvement. This study utilized an argon-based ARTP to conduct the mutations on SPR20. The positive mutants of 40% occurred. The M27 mutant exhib-ited an increase in anti-MRSA activity when compared to the wild-type, by which the MIC values were 250500 and 500 g/mL, respectively. M27 had genetic stability because it exhibited con-stant activity throughout 15 generations. This mutant had similar morphology and antibiotic susceptibility to the wild-type. Comparative proteomic analysis identified some specific proteins that were upregulated in M27. These proteins were involved in the metabolism of amino acids, cell structure and movement, and catalytic enzymes. These might result in the enhancement of the anti-MRSA activity of the ARTP-treated SPR20 mutant. This study supports the ARTP technology to increase the production of valuable antibacterial agents.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Brevibacillus Sp.

SUBMITTER: Sucheewin Krobthong

LAB HEAD: Apichart Atipairin

PROVIDER: PXD043954 | Pride | 2023-08-31

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	M27_1.raw	Raw
	M27_1_01.raw	Raw
	M27_1_02.raw	Raw
	M27_2_02.raw	Raw
	M27_3_01.raw	Raw

Items per page:

1 - 5 of 13

Similar Datasets

Transcriptome of Staphylococcus aureus Response to Spermine

2013-01-03 | GSE43230 | GEO

Anti-methicillin-resistant Staphylococcus aureus Peptides

Project description:Methicillin-resistant Staphylococcus aureus (MRSA) is a highly prioritized pathogen proposed by World Health Organization (WHO). It is listed to encourage researchers to search for effective antimicrobial agents. Previously, we isolated soil Brevibacillus sp. strain SPR19, which showed anti-MRSA activity. However, the active substances were still unknown. This study aimed to isolate and identify the anti-MRSA substances from this bacterium. The cell-free supernatant of SPR19 was subjected to salt precipitation, cation exchange, and re-versed-phase chromatography. Bioassay-guided fractionation was used to screen active sub-stances

2023-08-31 | PXD042035 | Pride

Transcription profiling by array of E. coli NCTC 9001 challenged with four different cationic antimicrobial peptides

Project description:Obtaining an in depth understanding of the arms races between peptides comprising the innate immune response and bacterial pathogens is of fundamental interest and will inform the development of new antibacterial therapeutics. Many cationic antimicrobial peptides (AMPs) share a range of structural and physical features that have been linked to antibacterial activity and yet they vary dramatically in their potency towards the same bacterial target. We hypothesised that a whole organism view of AMP challenge on Escherichia coli could provide a sophisticated, bacterial perspective enabling understanding of how potency is linked to mode of action. We used a 1H NMR metabolomic approach to characterise the effect on E. coli of challenge with four structurally and physically related AMPs: magainin 2, pleurocidin, buforin II and a designed peptide comprising D-amino acids only. Sub-inhibitory conditions, where these peptides nevertheless induced a bacterial response, were identified enabling electron microscopic and transcriptomic analyses. Although some common features of the bacterial response to AMP challenge could be identified, the metabolomes, morphological changes and the vast majority of the changes in gene expression were specific to each AMP. We show the antibacterial mode of action of AMPs can be accurately predicted by comparing ontological profiles generated by transcriptomic analyses. The response of E. coli to AMP challenge is highly plastic, with the bacteria capable of deploying a multifaceted response adapted to the mode of action rather than the physical properties of the AMP.

2014-01-21 | E-MTAB-1703 | biostudies-arrayexpress

Molecular Docking and Proteomics Reveals the Synergistic Antibacterial Mechanism of Theaflavin with β-lactam Antibiotics Against MRSA

Project description:Recurrent epidemics of methicillin-resistant Staphylococcus aureus (MRSA) have illustrated that the effectiveness of antibiotics in clinical application is rapidly fading. A feasible approach is to combine natural products with existing antibiotics to achieve an antibacterial effect. In this molecular docking study, we found that theaflavin (TF) preferentially binds the allosteric site of penicillin-binding protein 2a (PBP2a), inducing the PBP2a active site to open, which is convenient for β-lactam antibiotics to treat MRSA infection, instead of directly exerting antibacterial activity at the active site. Subsequent TMT-labeled proteomics analysis showed that TF treatment did not significantly change the landscape of the Staphylococcus aureus (S. aureus) USA300 proteome.Checkerboard dilution tests and kill curve assays were performed to validate the synergistic effect of TF and ceftiofur, and the fractional inhibitory concentration index (FICI) was 0.1875.Our findings provide a potential therapeutic strategy to combine existing antibiotics with natural products to resolve the prevalent infections of multidrug-resistant pathogens.

2022-11-09 | PXD037668 | Pride

Resistant isolates of methicillin-resistant Staphylococcus aureus (MRSA)

Project description:Resistant isolates of methicillin-resistant Staphylococcus aureus (MRSA) often cause infections with high rates of mortality. Antimicrobial peptides are source of molecules for new antimi-crobials development, such as melittin, a fraction of venom from Apis mellifera bee. The aims of this work were to evaluate antibacterial and antibiofilm activity of melittin and its association with oxa-cillin (meltoxa) on MRSA isolates and to investigate mechanisms of action on MRSA by using proteomic analysis.

2023-05-25 | MSV000092035 | MassIVE

Transcriptome of Staphylococcus aureus Response to Spermine

Project description:To explore the Spermine(Spm)-based antibacterial targets in S. aureus, time course-dependent transcriptome analysis was conducted on Mu50 (MRSA) in the absence and presence of Spm. We conducted five independent microarray experiments in the absence (control) and the presence (experimental) of Spm. We calculated fold change as the ratio between the signal of untreated (control) and Spm-treated (experimental) cultures for 15, 30 and 60 min exposures.

2013-01-03 | E-GEOD-43230 | biostudies-arrayexpress

Integration of Metabolomics and Transcriptomics Reveals Changes in MRSA Exposed to Terpinen-4-ol

Project description:Staphylococcus aureus (S. aureus) is a gram-positive bacterium that causes a wide range of diseases. Terpinen-4-ol is a monoterpene component contained in most plant essential oils with good antibacterial activity. In this study, terpinen-4-ol effectively inhibited 13 strains of S. aureus, and effectively inhibited the biofilm of MRSA. Metabolomics and transcriptomics were used to elucidate changes in MRSA cells exposed to terpinen-4-ol. Terpinen-4-ol significantly changed (greater than a 2- or less than a 2-fold change) the expression of 304 genes and the level of 847 metabolites (greater than a 1.5- or less than a 0.67-fold change) in MRSA. The levels of genes and metabolites related to the valine, leucine and isoleucine biosynthesis metabolism pathway, the purine and pyrimidine metabolism pathway, energy metabolism and β-lactam resistance were dramatically changed in biofilms exposed to terpinen-4-ol. To the best of our knowledge, this research is the first to report the metabolite and expression profiles of MRSA exposed to terpinen-4-ol.

2020-09-09 | GSE157638 | GEO

Quantitative analysis of the antibacterial effect of cinnamaldehyde on Staphylococcus aureus based on TMT protein

Project description:TMT quantitative proteomics was used to analyze the changes of protein expression in MRSA under the action of CA, and the antibacterial mechanism of CA on MRSA was further revealed.

2024-08-14 | PXD054851 |

Comparison of the oxacillin stress response in vraS and vraT mutants

Project description:To determine the role of the second gene in the vra operon (vraT) in the stress response to oxacillin, we compared the oxacillin induction profiles in a vraT deletion strain to that of a vraSR mutant in a USA300 MRSA strain background. Nonpolar deletion of vraT and insertional inactivation of vraS was constructed in MRSA strain USA300.

2012-11-30 | E-GEOD-39627 | biostudies-arrayexpress

Role of the accessory gene regulator agr in community-associated MRSA pathogenesis

Project description:The success of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) as pathogens is due to a combination of antibiotic resistance with high virulence. However, evolution of the exceptional virulence potential of CA-MRSA is not understood. Our previous study indicated that differential gene expression contributes substantially to this process. Thus, we here investigated the role of the pivotal virulence gene regulatory system agr in the most prevalent CA-MRSA strain USA300. Using a mouse subcutaneous infection model, we show that agr is essential for the development of CA-MRSA skin infections, the most frequent manifestation of disease caused by CA-MRSA. Furthermore, genome-wide analysis of gene expression revealed significant differences in agr-dependent virulence gene regulation between CA-MRSA, HA-MRSA, and laboratory strains. Our findings demonstrate that agr functionality is critical for CA-MRSA disease and indicate that an adaptation of the agr regulon to optimize expression of a broad set of virulence determinants may have contributed to the evolution of exceptionally pronounced virulence of CA-MRSA strains. Keywords: wild type vs mutant Wild type vs mutant agr strains.

2011-03-29 | E-GEOD-18793 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data