Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic Signature Profiling in the Cortex of Dairy Cattle Un-ravProteomic Signature Profiling in the Cortex of Dairy Cattle Un-ravels the Physiology of Brain Aging els the Physiology of Brain Aging

ABSTRACT: Aging is a physiological process occurring in all living organisms. It is characterized by a pro-gressive deterioration of the physiological and cognitive functions of the organism, accompanied by a gradual impairment of mechanisms involved in the regulation of tissue and organ homeo-stasis, thus exacerbating the risk of developing pathologies, including cancer and neurodegener-ative disorders. In the present work, we sought to investigate the influence of aging upon the expression protein profile in the brain cortex of grazing cattle, through LC-MS/MS-based differ-ential proteomics and bioinformatic analysis approach. We found a significant downregulation of proteins involved in synaptic plasticity, myelination, dendritic process, and oxidative stress in the cortex of aged bovine when compared to the adult samples. In conclusion, our data pave the way for a better understanding of molecular mechanisms underlying brain aging in cattle.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Brain

SUBMITTER: Flora Cozzolino

LAB HEAD: Maria Monti

PROVIDER: PXD044108 | Pride | 2023-11-27

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	CORTEX_AD1_REP1.raw	Raw
	CORTEX_AD1_REP2.raw	Raw
	CORTEX_AD2_REP1.raw	Raw
	CORTEX_AD2_REP2.raw	Raw
	CORTEX_AD3_REP1.raw	Raw

Items per page:

1 - 5 of 22

Similar Datasets

Proteomic signature profiling in the cortex of dairy cattle unravels the physiology of brain aging.

Project description:IntroductionAging is a physiological process occurring in all living organisms. It is characterized by a progressive deterioration of the physiological and cognitive functions of the organism, accompanied by a gradual impairment of mechanisms involved in the regulation of tissue and organ homeostasis, thus exacerbating the risk of developing pathologies, including cancer and neurodegenerative disorders.MethodsIn the present work, for the first time, the influence of aging has been investigated in the brain cortex of the Podolica cattle breed, through LC-MS/MS-based differential proteomics and the bioinformatic analysis approach (data are available via ProteomeXchange with identifier PXD044108), with the aim of identifying potential aging or longevity markers, also associated with a specific lifestyle.Results and discussionWe found a significant down-regulation of proteins involved in cellular respiration, dendric spine development, synaptic vesicle transport, and myelination. On the other hand, together with a reduction of the neurofilament light chain, we observed an up-regulation of both GFAP and vimentin in the aged samples. In conclusion, our data pave the way for a better understanding of molecular mechanisms underlying brain aging in grazing cattle, which could allow strategies to be developed that are aimed at improving animal welfare and husbandry practices of dairy cattle from intensive livestock.

| S-EPMC10733460 | biostudies-literature

Dairy Cattle Manure

Project description:Dairy Cattle Manure Raw sequence reads

| PRJNA701469 | ENA

Brain phosphoproteomics in aging without AD

Project description:(Phospho)proteomic studies in the human aging brain are based on the comparison between controls of bain aging usually affected by early stages of neurofibrillary tangle (NFT) pathology and subjects with moderate or advanced Alzheimer’s disease (AD). (Phospho)proteomics using conventional label‐free‐ and SWATH‐MS (Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry) has been assessed in the frontal cortex (FC) of individuals without NFTs and SPs, and comorbidities classified by age (years) in four groups; group 1 (young, 30-44); group 2 (middle-aged: MA, 45-52); group 3 (early-elderly, 64-70); and group 4 (late-elderly, 75-85).

2023-08-11 | PXD035997 | JPOST Repository

miRNA expression data from post-partum dairy cattle (Holstein Friesian) liver

Project description:High yielding dairy cattle undergo a state of NEB (negative energy balance) during the post-partum period when energy demand for lactation and maintenance exceeds energy intake. During this period in order to counteract NEB the liver under goes extensive metabolic and physiological change resulting in alteration in hepatic genes and miRNAs expression. We used Affymetrix Multispecies miRNA-2_0 Array with miRBase version 15 coverage to assess the liver miRNA expression in SNEB (severe NEB) and MNEB (mild NEB) Holstein Friesian cattle during the post-partum period. A NEB model of Holstein Friesian was established such that 12 post-partum cattle were randomly assigned to MNEB and SNEB groups depending on different feeding and milking regimes

2013-10-25 | E-GEOD-51658 | biostudies-arrayexpress

Whole transcriptome sequencing reveals dynamic RNA changes in the aging rat brain

Project description:Using RNA-seq, 9 cerebral cortex RNA samples were sequenced from 6 month (n=3, 6 individuals pooled), 12 month (n=3, 6 individuals pooled) and 28 month (n=3, 6 individuals pooled) rats. Allowing brain aging in the cerebral cortex to be assessed. Transcriptional profiling of the ageing cerebral cortex at 6, 12 and 28 months

2012-05-09 | E-GEOD-34272 | biostudies-arrayexpress

dairy cattle rumen metagenome

Project description:dairy cattle rumen metagenome Raw sequence reads

| PRJNA909074 | ENA

dairy cattle rumen metagenome

Project description:dairy cattle rumen metagenome Raw sequence reads

| PRJNA907195 | ENA

dairy cattle feces 16S

Project description:dairy cattle feces 16S Raw sequence reads

| PRJNA933720 | ENA

miRNA expression data from post-partum dairy cattle (Holstein Friesian) liver

2013-10-25 | GSE51658 | GEO

Gene expression profiles underlying aggressive behavior in the prefrontal cortex of cattle

Project description:This study compared gene expression profiles of 16 prefrontal cortex (PFC) samples from aggressive and non-aggressive cattle breeds: Lidia, selected for agonistic responses, and Wagyu, specialized on meat production and selected for tameness. RNA sequencing was used to identify and classify 918 up and 278 down-regulated differentiated expressed genes (DEG). The functional interpretation of the up-regulated genes in the aggressive cohort revealed enrichment of pathways such as the Alzheimer disease-presenilin, integrins or the ERK/MAPK signaling cascade, all implicated in the development of abnormal aggressive behaviors and neurophysiological disorders. Moreover, gonadotropins, leading to testosterone release, are also up-regulated as a natural mechanisms enhancing aggression. Concomitantly, heterotrimeric G-protein pathways, associated with low reactivity mental states, and the GAD2 gene, a repressor of agonistic reactions at the PFC, are down-regulated, guaranteeing the development of the adequate responses required by the aggressive Lidia cattle. We also identified six upstream regulators, whose functional activity fits with the etiology of abnormal behavioral responses associated with aggression.

2020-10-13 | GSE148938 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data