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Stomatal opening under high temperatures is controlled by the OST1-regulated TOT3–AHA1 module

ABSTRACT: Plants continuously respond to changing environmental conditions to prevent damage and maintain optimal performance. To regulate gas exchange with the environment and to control abiotic stress relief, plants have pores in their leaf epidermis, called stomata. Multiple environmental signals affect the opening and closing of these stomata. High temperatures promote stomatal opening (to cool down), and drought induces stomatal closing (to prevent water loss). Coinciding stress conditions may evoke conflicting stomatal responses, but the cellular mechanisms to resolve these conflicts are unknown. Here we demonstrate that the high-temperature-associated kinase TARGET OF TEMPERATURE 3 directly controls the activity of plasma membrane H+-ATPases to induce stomatal opening. OPEN STOMATA 1, which regulates stomatal closure to prevent water loss during drought stress, directly inactivates TARGET OF TEMPERATURE 3 through phosphorylation. Taken together, this signalling axis harmonizes stomatal opening and closing under high temperatures and drought. In the context of global climate change, understanding how different stress signals converge on stomatal regulation allows the development of climate-change-ready crops.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Shoot

SUBMITTER: Xiangyu Xu

LAB HEAD: Ive De

PROVIDER: PXD044300 | Pride | 2024-11-12

REPOSITORIES: Pride

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			Action	DRS
	Drought_phosphoproteome_Experiment_1_search_output.zip	Other
	Drought_phosphoproteome_experiment_1_Sample_info.xlsx	Xlsx
	Exp1_E28121_1p_50uPAC13__trap9_Col-0_W2.raw	Raw
	Exp1_E28123_1p_50uPAC13__trap9_Col-0_W3.raw	Raw
	Exp1_E28125_1p_50uPAC13__trap9_Col-0_W1.raw	Raw

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