Proteomics

Dataset Information

0

TAP-MS of human BRAT1 and WDR73


ABSTRACT: Using a combination of proteomics, interaction studies and structural characterization we show that WDR73 and BRAT1 are interactors of the heterodimer INTS9-11 of INT. Both proteins are involved in the stability and formation of the cleavage module of INT consisting of INTS4-9-11.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Kevin Sabath  

LAB HEAD: Dr. Stefanie Jonas

PROVIDER: PXD044324 | Pride | 2024-07-31

REPOSITORIES: Pride

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Publications

Assembly mechanism of Integrator's RNA cleavage module.

Sabath Kevin K   Qiu Chunhong C   Jonas Stefanie S  

Molecular cell 20240719 15


The modular Integrator complex is a transcription regulator that is essential for embryonic development. It attenuates coding gene expression via premature transcription termination and performs 3'-processing of non-coding RNAs. For both activities, Integrator requires endonuclease activity that is harbored by an RNA cleavage module consisting of INTS4-9-11. How correct assembly of Integrator modules is achieved remains unknown. Here, we show that BRAT1 and WDR73 are critical biogenesis factors  ...[more]

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