Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

LC-MS/MS detection of proteins involved in Escherichia coli biomineralized nanoparticles

ABSTRACT: Biomineralization is a naturally occurred process, by which microorganism reduced mental ions to minerals. Bacteria-driven biomineralization is most applied in metal recycling or environmental governance, the biomineralized products are rarely used. This probably due to the attachments of microorganism derived components on minerals, such as proteins, which are treated as impurities and hard to remove. However, these microorganism generated molecules are potent in activation of immune systems, suggesting promising potentials of biomineralized products in developing immunotherapeutic strategies. In this research, we analyzed the protein components on DH5a Escherichia coli produced gold nanoparticles, to explored the generation process of gold nanoparticles in bacterial cells, as well as its immune adjuvant potentials.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Escherichia Coli

TISSUE(S): Escherichia Coli Metabolite

DISEASE(S): Disease Free

SUBMITTER: Hao Qin

LAB HEAD: Guangjun Nie

PROVIDER: PXD044359 | Pride | 2023-08-25

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Lumos00575-KL-20190625-AU-OMV.raw	Raw
	Proteome.xlsx	Xlsx

Items per page:

1 - 2 of 2

Similar Datasets

Investigation of Alterations in Gene Expression in the Retina Induced by Intravitreal Injection of gold and silicate nanoparticles

Project description:In this study, we investigated the gene expression induced by locally delivered gold and silicate nanoaprticles with the diameter of 20 and 100 nm in the retina. We injected nanoparticles into the vitreous cavity of 5-week-old male C57BL/6 mice. Au20 indicates gold nanoparticles of which diameters were 20 nm, Au100 gold nanoparticles of which diameters were 100 nm, Si20 silicate nanoparticles of which diameters were 20 nm, and Si100 silicate nanoparticles of which diameters were 100 nm. We intravitreally injected PBS or nanoparticles (gold and silicate) into the right eyes of 5-week-old male C57BL/6 mice (n = 12 per group). PBS-treated mice were regarded as negative control. Four retinal tissues were pooled into 1 test tube and prepared for further analyses.

2013-07-31 | E-GEOD-49371 | biostudies-arrayexpress

Investigation of Alterations in Gene Expression in the Retina Induced by Intravitreal Injection of gold and silicate nanoparticles

2013-07-31 | GSE49371 | GEO

High antimony resistance strain Enterobacter sp. Z1 mediates biomineralization of antimony trioxide

Project description:Enterobacter sp. Z1 showed resistance to antimony and strong ability to produce antimony trioxide (Sb2O3) minerals. Thus, so the purpose of this project is to clarify mechanism of the biomineralization in strain Z1.

2024-03-21 | PXD050880 |

SM5-1-conjugated gold nanoparticles for antibody therapy in hepatocellular carcinoma

Project description:As a humanized mouse antibody, SM5-1 can target a membrane protein of about 230kDa over-expressed in hepatocellular carcinoma (HCC), melanoma and breast cancer and it has been found to inhibit the progress of tumor cells. In this study, SM5-1 conjugated gold nanoparticles were prepared to study the antitumor efficacy in the treatment of HCC-LM3-fLuc tumor. The results showed that AU-SM5-1 could inhibit HCC-LM3 hepatocellular carcinoma cell proliferation up to 71.26% at the concentration of 0.5mg/ml contrast with SM5-1 and gold nanoparticles. In order to address the mechanism of the antiproliferative effects of AU-SM5-1, we examined the gene expression in HCC-LM3-fLuc tumor cells based on gene-chip screening. The gene chip results showed that some cycle-related and reactive oxygen species (ROS) related genes including up-regulated P21 and down-regulated duox2 and nox1 genes which were validated by real-time quantitative polymerase chain reaction (PCR).

2014-07-11 | E-GEOD-59284 | biostudies-arrayexpress

Nano gold simulated autophagy prevents cell death

Project description:We showed a kind of rectangular shaped gold-based nanoparticles (gold nanocages, GNC) could enhance the survival of cells from hydroxyurea induced cell apoptosis and significantly reduced the reactive oxygen species (ROS) levels.The nanoparticles could initiate single layer isolation membrane elongation after been phagocytosed, followed by autophagosome and autolysosome formation, then specifically integrated into the autophagosome inner membrane and bind to p62. Through recycling ribosome proteins and activate Nrf2 mediated activation of GATA3 signaling, rescue the cell death and enhanced cell and life survival.

2020-05-09 | GSE131033 | GEO

RNA SEQUENCING DATA OF NOVEL GOLD NANOPARTICLE TREATED PANCREATIC CANCER CELLS.

Project description:BxPC3 pancreatic cancer cell lines were treated with Gold Nanoparticles and RNA was isolated from untreated and treated cells, RNA was sequences by NGS method and data reported

2024-05-17 | GSE248677 | GEO

Transcriptomic response in gilthead sea bream (Sparus aurata) liver exposed to gold nanoparticles

Project description:Explore the underlying mechanisms-of-action after short-term (24 h) waterborne exposure to low (0.5 μg/L) and high (50 μg/L) gold nanoparticles (AuNP) concentrations in gilthead sea bream.

2017-01-24 | GSE93930 | GEO

in vivo neurogenesis based electromagnetized gold nanoparticles

Project description:in vivo neurogenesis based electromagnetized gold nanoparticles

| PRJNA702647 | ENA

STRT RNAseq of THP-1 monocytic cells exposed to surface modified-gold nanoparticles

Project description:Transcriptome comparison to assess the responses of human monocytic cells (THP-1) to gold-nanoparticles (Au-NPs) of two different diameter sizes (5 or 20 nm) with different surface functional groups, i.e., alkylammonium bromide, alkyl sodium carboxylate, or poly(ethylene glycol) (PEG)-terminated thiolate Au-NPs.

2019-06-04 | E-MTAB-7093 | biostudies-arrayexpress

SM5-1-conjugated gold nanoparticles for antibody therapy in hepatocellular carcinoma

2014-07-11 | GSE59284 | GEO