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N-terminome analysis of tomato P69D knock-out mutant total leaf proteomes treated with and without recominant P69D

ABSTRACT: The intercellular space or apoplast constitutes the main interface in plant-pathogen interactions. Apoplastic subtilisin-like proteases -subtilases- may play an important role in defence and they have been identified as targets of pathogen-secreted effector proteins. However, most evidence is limited to the interaction of plants with non-vascular foliar pathogens. Here, we characterise the role of the Solanaceae-specific P69 subtilase family in the interaction between tomato and the vascular bacterial wilt pathogen Ralstonia solanacearum.

INSTRUMENT(S): impact II

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Leaf

SUBMITTER: Pitter Huesgen

LAB HEAD: Pitter Huesgen

PROVIDER: PXD044637 | Pride | 2023-12-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20221130_Weiqi_SOLLC_PR1proteins_reannotated.fasta	Fasta
	20221201_MQ_HUNTER_P69D_vs_EV_invitro_APO.7z	Other
	2022_04_RALSO_UP000001436_267608.fasta	Fasta
	20230712_MQ_HUNTER_total_invitro_shorterP69.7z	Other
	2023_03_UP000004994_4081_removed_A0A3Q7HVI0_A0A3Q7HXW1.fasta	Fasta

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Comparison of tomato wild type and P69D knock-out mutant apoplast proteome and N-terminomes

Project description:The intercellular space or apoplast constitutes the main interface in plant-pathogen interactions. Apoplastic subtilisin-like proteases -subtilases- may play an important role in defence and they have been identified as targets of pathogen-secreted effector proteins. However, most evidence is limited to the interaction of plants with non-vascular foliar pathogens. Here, we characterise the role of the Solanaceae-specific P69 subtilase family in the interaction between tomato and the vascular bacterial wilt pathogen Ralstonia solanacearum.

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