Proteomics

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Global-scale enrichment and identification of O-GlcNAc modified peptides in Arabidopsis seedling (S1 Part 1: HCD and EThcD part from Eclipse)

ABSTRACT: O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. However, our understanding of this PTM in plants lags behind animals. To bridge this knowledge gap, we performed a global scale enrichment of O-GlcNAc modified peptides after LWAC enrichment on Col seedlings. A 15N-labeled spy-4 is also included in the samples for comparison. Enriched samples were subjected to extensive reverse high pH fractionation followed by LC-MS-MS analysis on Thermo Fisher Orbitrap mass spectrometer (QExactive HF) using HCD modes (Files initiate with Q) or on Thermo Fisher Orbitrap mass spectrometer Eclipse with ETD using HCD 204 triggered EtHCD.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Shoot

SUBMITTER: Shouling Xu  

LAB HEAD: SHOULING XU

PROVIDER: PXD044701 | Pride | 2024-05-21

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
E20200929_01.pep.xml Pepxml
E20200929_01.raw Raw
E20200929_01F.pep.xml Pepxml
E20200929_01eM1.pep.xml Pepxml
E20200929_01eM2.pep.xml Pepxml
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Publications

SECRET AGENT O-GlcNAcylates Hundreds of Proteins Involved in Diverse Cellular Processes in Arabidopsis.

Shrestha Ruben R   Karunadasa Sumudu S   Grismer TaraBryn S TS   Reyes Andres V AV   Xu Shou-Ling SL  

Molecular & cellular proteomics : MCP 20240208 4

O-GlcNAcylation is a critical post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. While considerable knowledge exists about over 3000 substrates in animals, our understanding of this modification in plants remains limited. Unlike animals, plants possess two putative homologs: SECRET AGENT (SEC) and SPINDLY, with SPINDLY also exhibiting O-fucosylation activity. To investigate the role of SEC as a major O-GlcNAc transferas  ...[more]

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