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STPP-UP: A alternative method for drug target identification using protein thermal stability

ABSTRACT: Thermal proteome profiling (TPP) has significantly advanced the field of drug discovery by facilitating proteome-wide identification of drug targets and off-targets. However, TPP has not been widely applied for high-throughput drug screenings, since the method is labor intensive and requires a lot of measurement time on a mass spectrometer. Here, we present Single-tube TPP with Uniform Progression (STPP-UP), which significantly reduces both the amount of required input material and measurement time, while retaining the ability to identify drug targets for compounds of interest.

INSTRUMENT(S): Q Exactive Plus, Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture, Lymphoblast

DISEASE(S): Colorectal Cancer

SUBMITTER: Dick Zijlmans

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD044724 | Pride | 2023-10-24

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20230808_Exploris1_SA_DZ103_fraction2.raw	Raw
	20230808_Exploris1_SA_DZ103_fraction5.raw	Raw
	20230808_Exploris1_SA_DZ103_fraction7.raw	Raw
	20230808_Exploris1_SA_DZ103_fraction8.raw	Raw
	STPP-UP_C1_alternate_heating.rar	Other

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STPP-UP: A alternative method for drug target identification using protein thermal stability

Project description:Thermal proteome profiling (TPP) has significantly advanced the field of drug discovery by facilitating proteome-wide identification of drug targets and off-targets. However, TPP has not been widely applied for high-throughput drug screenings, since the method is labor intensive and requires a lot of measurement time on a mass spectrometer. Here, we present Single-tube TPP with Uniform Progression (STPP-UP), which significantly reduces both the amount of required input material and measurement time, while retaining the ability to identify drug targets for compounds of interest.

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