Dataset Information

Feed-forward stimulation of CAMK2 by the oncogenic pseudokinase PEAK1 generates an ‘actionable’ signalling axis in triple negative breast cancer

ABSTRACT: To characterize the interactomes of PEAK1 and PEAK2 homodimers, and the PEAK1/PEAK2 heterodimer, we applied bimolecular complementation affinity purification coupled with tandem mass spectrometry (BiCAP-MS/MS), a recently-developed technique that specifically identifies the interactors of any pair of interacting proteins while excluding those binding to individual components. Appropriate pairs of V1- and V2-tagged proteins were co-transfected into HEK293T cells, and then specific dimeric complexes were affinity purified with GFP-Trap nanobody and associated proteins identified by LC-MS/MS. Proteins exhibiting significantly increased abundance in particular PEAK dimers versus the Venus control were identified by label-free quantitative MS analysis, and are presented in volcano plots.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Xue Yang

LAB HEAD: Roger John Daly

PROVIDER: PXD044872 | Pride | 2025-01-04

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	20161129_bicap_01_V1.raw	Raw
	20161129_bicap_01_V1_DDA.raw	Raw
	20161129_bicap_02_V2.raw	Raw
	20161129_bicap_03_V3.raw	Raw
	20161129_bicap_04_223_1.raw	Raw

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Publications

Feed-forward stimulation of CAMK2 by the oncogenic pseudokinase PEAK1 generates a therapeutically "actionable" signalling axis in triple negative breast cancer.

Yang Xue X Ma Xiuquan X Zhao Tianyue T Croucher David R DR Nguyen Elizabeth V EV Clark Kimberley C KC Hu Changyuan C Latham Sharissa L SL Bayly-Jones Charles C Nguyen Viet Chi Bao VCB Budnar Srikanth S Shin Sungyoung S Nguyen Lan K LK Cotton Thomas R TR Chueh Anderly C AC Sian Terry Cc Lim Kam TCLK Stratton Margaret M MM Ellisdon Andrew M AM Daly Roger J RJ

bioRxiv : the preprint server for biology 20241122

The PEAK family of pseudokinases, comprising PEAK1-3, are signalling scaffolds that play oncogenic roles in several poor prognosis human cancers, including triple negative breast cancer (TNBC). However, therapeutic targeting of pseudokinases is challenging due to their lack of catalytic activity. To address this, we screened for PEAK1 effectors by affinity purification and mass spectrometry, identifying calcium/calmodulin-dependent protein kinase 2 (CAMK2)D and CAMK2G. PEAK1 promoted CAMK2D/G ac ...[more]

PMID: 38405732

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