Cysteine-rich with EGF-like domains 2 (CRELD2) is an endoplasmic reticulum stress-inducible angiogenic growth factor promoting ischemic heart repair
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ABSTRACT: Tissue repair after myocardial infarction (MI) is guided by incompletely defined autocrine and paracrine-acting proteins. Deciphering these signals and their upstream triggers is essential when considering infarct healing as a therapeutic target. Searching for previously unknown growth factors involved in infarct repair, we performed a bioinformatic secretome analysis in cardiac endothelial cells and identified cysteine-rich with EGF-like domains 2 (CRELD2), an endoplasmic reticulum stress-inducible protein with poorly characterized function. CRELD2 was abundantly expressed and secreted in the heart after MI in mice and patients. Creld2-deficient mice and wild-type mice treated with a CRELD2-neutralizing antibody displayed impaired de novo microvessel formation in the infarct border zone and developed severe postinfarction heart failure. CRELD2 protein therapy, conversely, improved heart function after MI. Exposing human coronary artery endothelial cells to recombinant CRELD2 induced angiogenesis, associated with a distinct phosphoproteome signature. These findings identify CRELD2 as a novel angiogenic growth factor and unravel a link between endoplasmic reticulum stress and ischemic tissue repair.
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cardiac Endothelial Cell, Cell Culture
DISEASE(S): Myocardial Ischemia
SUBMITTER: Felix Polten
LAB HEAD: Kai Christoph Wollert
PROVIDER: PXD045013 | Pride | 2023-11-30
REPOSITORIES: pride
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