Proteomics

Dataset Information

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O-GlcNAcylation in mitochondrial protein in rat astrocytes


ABSTRACT: To further investigate this idea that O-GlcNAcylation in mitochondria can improve the efficacy and benefit of mitochondrial transfer, we asked what mitochondrial proteins were modified with O-GlcNAc. To identify O-GlcNAcylated proteins and the potential target sites of O-GlcNAcylation, rat astrocytes were stimulated with NAD to induce O-GlcNAcylation in mitochondria, then we isolated mitochondria and extracted O-GlcNAcylated proteins by immunoprecipitation using O-GlcNAc antibody followed by silver staining.

INSTRUMENT(S): LTQ

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Fibroblast

DISEASE(S): Disease Free

SUBMITTER: Kazuhide Hayakawa  

LAB HEAD: Kazuhide Hayakawa

PROVIDER: PXD045316 | Pride | 2023-10-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
48685_47167.mzXML Mzxml
48685_73965.mzid.gz Mzid
48685_73965.pep.xml Pepxml
48685_out.tgz Other
48685_sequest.params Other
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