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Impact of media supplementation on a transcriptional activity of porcine embryos produced in vitro

ABSTRACT: The production of embryos in vitro caused a revolution in the field of assisted reproduction technologies, which currently allows the manipulation and selection of embryos before implantation. One of the key aspects of this process is the maturation of oocytes in a controlled in vitro environment. In this article, we focused on the impact of precisely chemically modified FLI maturation medium and its potential in improving the efficiency of in vitro production of porcine embryos. Based on our findings, we demonstrated the positive influence of FLI medium in the evaluation of in vitro maturation, in vitro embryo production, EGA detection, transcriptomic profile and proteomic analysis, which was confirmed by the positive activation of the embryonal genome in the 4blastoembry stage of parthenogenetically activated embryos.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Oocyte

SUBMITTER: Martin Morovic

LAB HEAD: Martin Morovic

PROVIDER: PXD045481 | Pride | 2023-09-25

REPOSITORIES: Pride

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			Action	DRS
	DMEM01.mzML	Mzml
	DMEM01.xls	Xls
	DMEM02.mzML	Mzml
	DMEM02.xls	Xls
	DMEM03.mzML	Mzml

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Tumor suppressor TAp73 is required to maintain pluripotency of cancer stem cells

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