Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Flash proteotyping of microbial isolates by tandem mass spectrometry

ABSTRACT: Flash proteotyping is a methodology for ultra-fast identification of microorganisns by tandem mass spectrometry. Here, we obtained results on five reference strains and ten new bacterial isolates. The methodology is based on direct sample infusion into the mass spectromete and an original, highly sensitive procedure for data processing and taxonomic identification.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Kocuria Rhizophila Roseomonas Mucosa Pseudomonas Zeshuii Staphylococcus Equorum Staphylococcus Warneri Ruegeria Pomeroyi Dss-3 Micrococcus Luteus (micrococcus Lysodeikticus) Deinococcus Proteolyticus Mrp Microvirga Ossetica Pseudomonas Psychrotolerans Paenibacillus Catalpae Klebsiella Aerogenes Kctc 2190 Pseudomonas Putida Kt2440

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD045510 | Pride | 2023-12-21

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E04878_F21_R1.mgf	Mgf
	E04878_F21_R2.mgf	Mgf
	E04878_F21_R3.mgf	Mgf
	E04878_F21_R4.mgf	Mgf
	E04878_F21_R5.mgf	Mgf

Items per page:

1 - 5 of 47

Similar Datasets

ASTRAL 60 and 90 min in-depth metaproteomics of a human fecal standard to evaluate depth of analysis following a taxonomical proteotyping strategy.

Project description:In-depth metaproteomics of a human fecal standard, MetaP, with ASTRAL tandem mass spectometer operated in data-independent acquisition mode for 60 min or 90 min and with a database built following sample taxonomical proteotyping.

2024-03-07 | PXD046320 | Pride

ASTRAL 15 and 30 min in-depth metaproteomics of a human fecal standard to evaluate depth of analysis following a taxonomical proteotyping strategy.

Project description:In-depth metaproteomics of a human fecal standard, MetaP, with ASTRAL tandem mass spectometer operated in data-independent acquisition mode for 15 min or 30 min and with a database built following sample taxonomical proteotyping.

2024-03-07 | PXD046290 | Pride

Tandem mass spectrometry proteotyping of microbial samples isolated from an industrial infrastructure.

Project description:Tandem mass spectrometry proteotyping of microbial samples isolated from an industrial infrastructure for identifying their species taxonomical rank.

2021-09-09 | PXD020976 | Pride

Pure and mixed crude extracts of Thauera aromatica K172 and Pseudomonas putida KT2440 for benchmarking GroEL-proteotyping

Project description:This dataset has been used to establish GroEL-proteotyping, a targeted proteotyping approach for assessing bacterial community compositions using the taxonomic marker protein GroEL. This dataset contains raw data of two experiments: 1. Pure cultures of T. aromatica (sample names: TA, TB, TC) and P. putida (sample names: PA, PB, PC) were analyzed individually after in-solution digestion 2. T. aromatica and P. putida were cultivated independently and crude extracts were mixed in defined ratios. GroEL was pre-separated by SDS-PAGE (sample names: LOD_..., in-gel digestion)

2023-10-27 | PXD046460 | Pride

Proteotyping of sets of six microbial isolates by tandem mass spectrometry.

Project description:Proteotyping of mixtures of isolates was carried out by shotgun proteomics and phylopeptidomic interpretation to identify the microorganisms at the species taxonomical rank.

2024-05-02 | PXD044755 | Pride

Paleoproteomics of ancient human teeth

Project description:Teeth are a well-known source of information for paleoanthropologists. Here, we established the ancient dental metaproteomes in several samples from historic sites. The shotgun metaproteomics analysis relies on a iterative search strategy for the identification of the proteins and their origins.

2023-03-30 | PXD031786 | Pride

Extended paleoproteomics of ancient human teeth

2023-03-30 | PXD031802 | Pride

Exploris 480 dataset acquired on a human fecal standard to evaluate metaproteomics strategies.

Project description:Metaproteomics of a human fecal standard, MetaP, with an Exploris480 tandem mass spectometer operated in data-dependent analysis for proteotyping and evaluation of metaproteomics strategies.

2024-03-07 | PXD047139 | Pride

High-throughput proteotyping based on microplate paramagnetic beads and phylopeptidomics

Project description:Tandem mass spectrometry-based proteotyping of a mixture of three microorganisms.

2022-04-23 | PXD017720 | Pride

ASTRAL deep-proteotyping of a human fecal standard to evaluate metaproteomics strategies.

Project description:Metaproteomics of a human fecal standard, MetaP, with ASTRAL tandem mass spectometer operated in data-dependent analysis for deep-proteotyping and evaluate metaproteomics strategies.

2024-03-07 | PXD045838 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data