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Plasticity of the leaf lipidome and proteome and in response to pathogen infection and heat stress

ABSTRACT: Plants must cope with a variety of different stressors during their life cycle and their adaptations to these environmental influences involves all cellular organelles. Among them, comparatively little is known about the contribution of cytosolic lipid droplets (LDs) and their core of neutral lipids and associated surface proteins to the rewiring of cellular processes in response to stress. Here, we analyzed the changes that occur in the lipidome and proteome of Arabidopsis leaves after pathogen infections with Botrytis cinerea and Pseudomonas syringae, and heat stress. Analyses were carried out in wild-type plants and the oil-rich double mutant tgd1-1 sdp1-4 that allowed an allied study of the LD proteome in stressed leaves. Using liquid chromatography-tandem mass spectrometry-based methods, we show that a hyperaccumulation of the primary LD core component triacylglycerol is a general response to stress and that acyl chains are remodeled during cellular adaptation. Likewise, comparative analysis of the LD protein composition in stress-treated leaves highlighted the remodeling of the LD proteome as part of the general stress response. We further identified two new LD-associated proteins whose localization to LDs in leaves was confirmed by confocal microscopy of fluorescent protein fusions. Taken together, these and other results highlight LDs as dynamic contributors to the cellular adaptation processes that underlie how plants respond to environmental stress.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Till Ischebeck

LAB HEAD: Till Ischebeck

PROVIDER: PXD045596 | Pride | 2025-03-21

REPOSITORIES: Pride

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Project description:The timing of reproductive development determines spike architecture and thus yield in temperate grasses such as barley (Hordeum vulgare L.). Reproductive development in barley is controlled by the photoperiod response gene Ppd-H1 which accelerates flowering time under long-day (LD) conditions. A natural mutation in Ppd-H1 prevalent in spring barley causes a reduced photoperiod response, and thus, late flowering under LD. However, it is not very well understood how LD and Ppd-H1 control pre-anthesis development, and thus spike architecture and yield in barley. We performed a detailed morphological analysis of the pre-anthesis development in the spring barley cultivar Scarlett and its wild barley derived introgression line S42-IL107, carrying the photoperiod responsive Ppd-H1 allele. Characterization of shoot apex development in these genotypes indicated that floral transition and initiation of floral primordia occurred under LD (16h light/ 8h dark) and short day (SD, 8h light/ 16h dark) conditions, while inflorescence and seed development strictly required LDs. Additionally, a fast photoperiod response in the presence of the dominant Ppd-H1 allele promoted floret fertility under LDs. To characterize the effects of the photoperiod and allelic variation at Ppd-H1 on gene expression during pre-anthesis development we performed RNA sequencing of leaves and developing main shoot apices during the vegetative phase and early stages of inflorescence development in Scarlett and S42-IL107 grown under SD and LD conditions. Main shoot apices of both genotypes were sampled at defined developmental stages, i.e. Waddington stage W0.5, W1.0, W2.0 and W3.5, respectively. Leaf samples were harvested from plants before (W1.0) and after floral transition (W2.0). We identified genes that were specifically regulated at floral transition independent of day-length and Ppd-H1 and thus may serve as markers for the staging of floral transition. Furthermore, we identified transcripts differentially expressed between photoperiods and between genotypes in leaves and in shoot apices. This set of transcripts might act as candidates downstream of Ppd-H1 and are correlated with the promotion of shoot apex development and higher floret fertility under LD and in the presence of the photoperiod responsive Ppd-H1 allele.

			Action	DRS
	MaxQuant_1.6.17.0.zip	Other
	Metadatafile.pdf	Pdf
	PS_Q_10.raw	Raw
	PS_Q_11.raw	Raw
	PS_Q_12.raw	Raw

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Plasticity of the leaf lipidome and proteome and in response to pathogen infection and heat stress

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