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Massive intein content in Anaeramoeba reveals new aspects of intein mobility in eukaryotes

ABSTRACT: Inteins are self-splicing protein elements found in viruses and all three domains of life. How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. Here we show that the nuclear genomes of three strains of Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryote described previously. The Anaeramoeba inteins reside in a wide range of proteins, only some of which correspond to intein-containing proteins in other eukaryotes, prokaryotes and viruses. Our data also suggest that viruses have contributed to the spread of inteins in Anaeramoeba and the colonization of new alleles. The persistence of Anaeramoeba inteins might be partly explained by intragenomic movement of intein-encoding regions from gene to gene. Our intein dataset greatly expands the spectrum of intein-containing proteins and provides insights into the evolution of inteins in eukaryotes.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Anaeramoeba Flamelloides

SUBMITTER: Jon Jerlström Hultqvist

LAB HEAD: Jon Jerlström

PROVIDER: PXD045607 | Pride | 2023-11-30

REPOSITORIES: Pride

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			Action	DRS
	4688_1FDRpeptideProtein_db3_1A_2A_3A_Try_Lumos_230609_230619_sent_headings.xlsx	Xlsx
	4688_1FDRpeptideProtein_db3_1B_2B_3B_ChymoTry_Lumos_230609_230619_sent_headings.xlsx	Xlsx
	4688_1FDRpeptideProtein_db4_1A_2A_3A_Try_Lumos_230609_230619_sent_headings.xlsx	Xlsx
	4688_1FDRpeptideProtein_db4_1B_2B_3B_ChymoTry_Lumos_230609_230619_sent_headings.xlsx	Xlsx
	4688_db3_1A_2A_3A_Try_Lumos_230609_230619.pdResult	Other

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Massive intein content in Anaeramoeba reveals new aspects of intein mobility in eukaryotes

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