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ANP32B is a macroh2A histone chaperone

ABSTRACT: Interaction proteomics in cell extracts on tagged macroH2A, either expressed in cells or using recombinant dimers with H2B.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Imke Mandemaker

LAB HEAD: Andreas Ladurner

PROVIDER: PXD045696 | Pride | 2023-10-24

REPOSITORIES: Pride

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			Action	DRS
	Ref2195_IM_IPTriton_20190513.raw	Raw
	Ref2195_IMandemaker_20190513.csv	Csv
	Ref2195_IMandemaker_20190513.sld	Other
	Ref3433_IM_01.raw	Raw
	Ref3433_IM_02.raw	Raw

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Publications

The histone chaperone ANP32B regulates chromatin incorporation of the atypical human histone variant macroH2A.

Mandemaker Imke K IK Fessler Evelyn E Corujo David D Kotthoff Christiane C Wegerer Andreas A Rouillon Clément C Buschbeck Marcus M Jae Lucas T LT Mattiroli Francesca F Ladurner Andreas G AG

Cell reports 20231019 10

All vertebrate genomes encode for three large histone H2A variants that have an additional metabolite-binding globular macrodomain module, macroH2A. MacroH2A variants impact heterochromatin organization and transcription regulation and establish a barrier for cellular reprogramming. However, the mechanisms of how macroH2A is incorporated into chromatin and the identity of any chaperones required for histone deposition remain elusive. Here, we develop a split-GFP-based assay for chromatin incorpo ...[more]

PMID: 37858472

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