Proteomics

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Comparison of proteins identified using the Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) versus the non-functional (G42E) Macro domain for enrichment of ADP-ribosylated proteins from A. thaliana plants expressing the Pseudomonas syringae type III effectors AvrRpm1 or HopF2 (DDA method)

ABSTRACT: ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana plants expressing the Pseudomonas syringae type III effectors AvrRpm1 or HopF2. A non-functional Af1521G42E mutant of the engineered Macro domain was used as control. Enriched proteins were analyzed by LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Lennart Wirthmueller  

LAB HEAD: Lennart Wirthmueller

PROVIDER: PXD045726 | Pride | 2023-09-28

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
KSI230223_A1_Af1521_4A_1ul_Triggered.raw Raw
KSI230223_A1_Af1521_4A_2ul_Triggered.raw Raw
KSI230223_A1_G42E_3A_1ul_Triggered.raw Raw
KSI230223_A1_G42E_3A_2ul_Triggered.raw Raw
S4_run1_KSI230223_A1_Af1521_4A_1ul_Triggered.msf Msf
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