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Immunoproximity biotinylation reveals the axon initial segment proteome

ABSTRACT: The axon initial segment (AIS) is a specialized neuronal compartment required for action potential generation and neuronal polarity. However, understanding the mechanisms regulating AIS structure and function has been hindered by an incomplete knowledge of its molecular composition. Here, using immuno-proximity biotinylation we further define the AIS proteome and its dynamic changes during neuronal maturation. Among the many AIS proteins identified, we show that SCRIB is highly enriched in the AIS both in vitro and in vivo, and exhibits a periodic architecture like the axonal spectrin-based cytoskeleton. We found that ankyrinG interacts with and recruits SCRIB to the AIS. However, loss of SCRIB has no effect on ankyrinG. This powerful and flexible approach further defines the AIS proteome and provides a rich resource to elucidate the mechanisms regulating AIS structure and function.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture

SUBMITTER: Wei Zhang

LAB HEAD: Peng Zou

PROVIDER: PXD045921 | Pride | 2024-01-02

REPOSITORIES: pride

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			Action	DRS
	10_plex_TMT1_DIV7_DIV14.zip	Other
	10_plex_TMT2_DIV21_DIV14.zip	Other
	1_041_ZW0125_210106_NF186.raw	Raw
	1_041_ZW0125_NF186_1230_EXP2.raw	Raw
	1_041_ZW0125_NF186_E1_1.raw	Raw

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