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Genetic components of Escherichia coli involved in its complex preypredator interaction with Myxococcus xanthus

ABSTRACT: Myxococcus xanthus and Escherichia coli represent a well-studied microbial predatorprey pair frequently examined in laboratory settings. While significant progress has been made in comprehending the mechanisms governing M. xanthus predation, various aspects of the response and defensive mechanisms of E. coli as prey remain elusive. In this study, the E. coli large-scale chromosome deletion library was screened, and a mutant designated as ME5012 was identified to possess remarkable resistance to predation by M. xanthus. Within the deleted region of ME5012 encompassing seven genes, the significance of dusB and fis genes in driving the observed resistant phenotype became apparent. Specifically, the deletion of fis resulted in a notable reduction in flagellum production in E. coli, contributing to a certain level of resistance against predation by M. xanthus. Meanwhile, the removal of dusB in E. coli led to diminished inducibility of myxovirescin A production by M. xanthus, accompanied by a slight increase in resistance to myxovirescin A. These findings shed light on the molecular mechanisms underlying the complex interaction between M. xanthus and E. coli in a predatory context.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Ning Zhang

LAB HEAD: Ning Zhang

PROVIDER: PXD046017 | Pride | 2024-01-26

REPOSITORIES: Pride

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Genetic components of Escherichia coli involved in its complex prey-predator interaction with Myxococcus xanthus.

Zhang Ning N Li Tingyi T Pan Hongwei H Wang Yipeng Y Li Qi Q Luan Jia J He Xuesong X Shi Wenyuan W Li Yuezhong Y Wang Chuandong C Zhang Fengyu F Hu Wei W

Frontiers in microbiology 20231205

Myxococcus xanthus and Escherichia coli represent a well-studied microbial predator-prey pair frequently examined in laboratory settings. While significant progress has been made in comprehending the mechanisms governing M. xanthus predation, various aspects of the response and defensive mechanisms of E. coli as prey remain elusive. In this study, the E. coli MG1655 large-scale chromosome deletion library was screened, and a mutant designated as ME5012 was iden ...[more]

PMID: 38116529

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Project description:Purpose: Protozoan predators affect the structure of bacterial communities, but investigations into how predation influences bacterial evolution and antagonistic behaviours are scarce. We performed a 20-day predator-prey evolution experiment on solid media to investigate the adaptive traits that arise in bacterial prey under continuous protozoan predation. Methods: Pseudomonas fluorescens SBW25 and a wild Acanthamoeba sp. isolate as a predator prey pair co-evolved for 20 days yielded both previously described (Wrinkly Spreader; WS) and novel colony morphotype (Wrinkly Fried Egg; WFE) isolates with conferred grazing resistance. These isolates were subjected to RNAseq profiling with and without predation to determine transcriptional changes contributing to grazing resistance. Results: For differential gene expression the WT SBW25 without predation was used as a baseline. For the WS condition, a total of 881 differentially expressed genes (DEGs) were identified, of which 424 were upregulated and 457 were downregulated. In the WFE condition, a total of 908 DEGs were identified, of which 475 were upregulated and 434 were downregulated. Among all DEGs, 335 upregulated and 313 downregulated genes were shared between the WS1 and WFE conditions Conclusions: Our findings suggest that protozoan predation can profoundly influence the course of genetic and phenotypic evolution in a short period of time. Together, the differential expression results suggest expression of features that would be expected to increase biofilm formation in WFE according to previous studies. However, increased expression of these traits may not lead to a stronger biofilm, but may still provide predation resistance. For example, fibrils may increase the effective profile size of a bacterial cell. Increased Fap-mediated biofilm formation also induces increased alginate synthesis in P. aeruginosa PA01, an exopolysaccharide that protects mucoid P. aeruginosa against macrophage killing. Interestingly, we found increased expression of alginate biosynthesis genes in both WFE and WS1 (algA, algF), suggesting alternate mechanisms leading to increased alginate production in these two strains.

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Dataset Information

Genetic components of Escherichia coli involved in its complex preypredator interaction with Myxococcus xanthus

Dataset's files

Publications

Genetic components of <i>Escherichia coli</i> involved in its complex prey-predator interaction with <i>Myxococcus xanthus</i>.

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