Dataset Information

Protein and cytokine profiles associated with COVID-19 disease severity in Puerto Rico

ABSTRACT: Viral strains, age, and host factors including genetics and proteins are associated with variable immune responses against SARS-CoV-2 and disease severity. We hypothesized that unique proteins/pathways are associated with COVID-19 disease severity in Puerto Rican Hispanics. A total of 121 men and women aged 21-80 years-old were recruited in Puerto Rico. Plasma samples were collected from unvaccinated COVID-19 infected subjects during acute disease (n=39) and compared to COVID-19 negative individuals (n=56) during acute disease using proteomics and cytokine expression. Infected individuals were stratified based on symptomatology as follows: mild (n=18), moderate (n=13), and severe (n=8). Quantitative proteomics was performed in plasma samples using Tandem Mass Tag (TMT) labeling. Cytokines in plasma were quantified using a human cytokine array. Proteomics analyses revealed 56 differentially regulated proteins and the top 3 pathways that were predicted to be inhibited in severe patients including LXR/RXR signaling, Production of NO and ROS in macrophages, and Synaptogenesis signaling. Decreased cadherin-13 validated by ELISA, which participates in synaptogenesis, is a novel protein is a novel protein not previously reported in other studies of COVID-19 severity and validated by ELISA. Cytokine analyses showed that TNF⍺ levels decreased with disease severity. This study uncovers potential host predictors of COVID-19 severity and new avenues for treatment in Puerto Rican Hispanics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood Plasma

DISEASE(S): Covid-19

SUBMITTER: Loyda Melendez

LAB HEAD: Loyda Melendez

PROVIDER: PXD046103 | Pride | 2024-06-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	TMT_Analysis_COVID-19_EX2_20Dic2021_rev.msf	Msf
	TMT_Analysis_COVID-19_EX2_20Dic2021_rev.mzML	Mzml
	TMT_Analysis_COVID-19_EX2_20Dic2021_rev.mzid.gz	Mzid
	TMT_Pool1A_Fraction1_COVID19_Exp2_9nov2021.raw	Raw
	TMT_Pool1A_Fraction2_COVID19_Exp2_9nov2021.raw	Raw

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Publications

Plasma Proteins Associated with COVID-19 Severity in Puerto Rico.

Rosario-Rodríguez Lester J LJ Cantres-Rosario Yadira M YM Carrasquillo-Carrión Kelvin K Rosa-Díaz Alexandra A Rodríguez-De Jesús Ana E AE Rivera-Nieves Verónica V Tosado-Rodríguez Eduardo L EL Méndez Loyda B LB Roche-Lima Abiel A Bertrán Jorge J Meléndez Loyda M LM

International journal of molecular sciences 20240516 10

Viral strains, age, and host factors are associated with variable immune responses against SARS-CoV-2 and disease severity. Puerto Ricans have a genetic mixture of races: European, African, and Native American. We hypothesized that unique host proteins/pathways are associated with COVID-19 disease severity in Puerto Rico. Following IRB approval, a total of 95 unvaccinated men and women aged 21-71 years old were recruited in Puerto Rico from 2020-2021. Plasma samples were collected from COVID-19- ...[more]

PMID: 38791465

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