Dataset Information

Xiphophorus Hybrid Liver Mitochondria PRM

ABSTRACT: Hybrids between species contain a mixture of two divergent proteomes, the combination of which may lead to dysfunctional protein-protein interactions. We performed an integrative study on hybrids between the swordtail fishes Xiphophorus malinche and Xiphophorus birchmanni, and found that the combination of X. birchmanni ndufs5 (a nuclear-encoded subunit of mitochondrial Complex I) with X. malinche mtDNA (which codes for multiple Complex I subunits) was lethal. We reasoned that if this lethality was due to dysfunctional protein-protein interactions in Complex I causing improper assembly, then in heterozygotes which possess one functional and one dysfunctional allele, the dysfunctional allele might be more likely to exist in an improperly integrated/folded state, and thus be more likely to be degraded by protein quality control mechanisms. This preferential degradation would be measurable as a bias in the proteome towards the compatible allele of ndufs5, and so we sought to measure the relative abundance of peptides derived from the X. malinche and X. birchmanni alleles of ndufs5 in hybrids heterozygous at ndufs5, with X. malinche ancestry in the mtDNA. Peptides derived from each allele are distinguishable by multiple amino acids, and we used heavy-labeled spike-ins to target multiple ndufs5 peptides from each species in 5 hybrids. We successfully detected one pair of birchmanni/malinche peptides, and compared their relative abundance using peak integration, then compared the ratio to that observed in the heavy-labeled spike-in, for which the true ratio was known. We found that the endogenous peptides were skewed towards the allele from the same species as the mtDNA (X. malinche), consistent with our hypothesis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Xiphophorus Malinche X Xiphophorus Birchmanni

SUBMITTER: Ben Moran

LAB HEAD: Molly E Schumer

PROVIDER: PXD046217 | Pride | 2024-05-23

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20210616_BMoran_Schumer_11490_PRM_F1_mito_1.raw	Raw
	20210616_BMoran_Schumer_11490_PRM_F1_mito_1.raw_20210617_Byonic.byparms	Raw
	20210616_BMoran_Schumer_11490_PRM_F1_mito_1.raw_20210617_Byonic.mgf	Mgf
	20210616_BMoran_Schumer_11490_PRM_F1_mito_1.raw_20210617_Byonic.mzid.gz	Mzid
	20210616_BMoran_Schumer_11490_PRM_F1_mito_2.raw	Raw

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Publications

A lethal mitonuclear incompatibility in complex I of natural hybrids.

Moran Benjamin M BM Payne Cheyenne Y CY Powell Daniel L DL Iverson Erik N K ENK Donny Alexandra E AE Banerjee Shreya M SM Langdon Quinn K QK Gunn Theresa R TR Rodriguez-Soto Rebecca A RA Madero Angel A Baczenas John J JJ Kleczko Korbin M KM Liu Fang F Matney Rowan R Singhal Kratika K Leib Ryan D RD Hernandez-Perez Osvaldo O Corbett-Detig Russell R Frydman Judith J Gifford Casey C Schartl Manfred M Havird Justin C JC Schumer Molly M

Nature 20240110 7997

The evolution of reproductive barriers is the first step in the formation of new species and can help us understand the diversification of life on Earth. These reproductive barriers often take the form of hybrid incompatibilities, in which alleles derived from two different species no longer interact properly in hybrids<sup>1-3</sup>. Theory predicts that hybrid incompatibilities may be more likely to arise at rapidly evolving genes<sup>4-6</sup> and that incompatibilities involving multiple gen ...[more]

PMID: 38200310

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