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SKBR3/HER2+ Breast Cancer Cellular Response to Brain Cell-Secreted Factors

ABSTRACT: To address the challenges associated with the investigation of brain-metastasized cancers and provide insights into the drivers of cancer cell behavior at the brain metastatic site, this study was aimed at exploring the response of SKBR3/HER2+ cells to neural factors secreted by brain cells that come first in contact with the metastasized cancer cells, i.e., human endothelial cells (HBEC5i), astrocytes (NHA), and microglia (HMC3). To this end, serum-starved breast cancer cells were exposed to the combined secretome of brain cells, allowed to grow for 24h, and analyzed by LC-MS/MS using an Orbitrap QE instrument to generate and compare the whole cell proteome profiles of treated vs non-treated cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Brain Cancer,Breast Cancer

SUBMITTER: Iulia M. Lazar

LAB HEAD: Iulia M Lazar

PROVIDER: PXD046330 | Pride | 2024-08-27

REPOSITORIES: Pride

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			Action	DRS
	SKBR3_Cyto_control_11.msf	Msf
	SKBR3_Cyto_control_11.raw	Raw
	SKBR3_Cyto_control_12.msf	Msf
	SKBR3_Cyto_control_12.raw	Raw
	SKBR3_Cyto_control_13.msf	Msf

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