Dataset Information

Does protein-bound methionine sulfoxide provides a proteomic basis for ageing in beech seeds?

ABSTRACT: Beech trees produce seeds irregularly, necessitating the storage of these seeds for forestation. Despite the acquisition of desiccation tolerance during development, beech seeds lose viability during long-term storage faster than orthodox-type seeds. Seeds stored for short (3 years) and long (20 years) periods under optimal conditions were compared. Aged seeds characterized with germination capacity reduced to 30% displayed increased membrane damage, manifested as electrolyte leakage and lipid peroxidation levels. Analyses have been based on embryonic axes containing higher levels of reactive oxygen species (ROS) and higher levels of protein-bound methionine sulfoxide (MetO) in aged seeds. Using gel-free shotgun proteomics, 3,949 proteins were identified and 2,442 were reliably quantified to indicate 25 proteins with upregulated abundance and 35 with downregulated abundance in beech seeds under long-term storage compared to those under short-term storage. Functional analyses based on gene ontology annotations revealed that nucleic acid binding activity (biological process), hydrolases (molecular function), ribosome organization or biogenesis and transmembrane transport (cellular processes), and ATP synthesis (pathway) were affected in aged seeds. To verify whether MetO the oxidative posttranslational modification of proteins reversed via the action of methionine sulfoxide reductase (Msr) enzymes is involved in ageing of beech seeds we identified 316 and reliably quantified 226 MetO-containing proteins, among which 9 and 19 exhibited significantly up- and downregulated MetO levels, respectively, in beech seeds under long-term storage. Several Msr isoforms were identified and recognized as MsrA1-like, MsrA4, MsrB5 and MsrB5-like in beech seeds. Only MsrA1-like displayed decreased abundance in aged seeds. We demonstrated that the loss of membrane integrity reflected in elevated abundance of membrane proteins had higher impact on seed ageing progress rather than MetO/Msr system.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Fagus Sylvatica

SUBMITTER: Sara Dufour

LAB HEAD: Ewa Marzena Kalemba

PROVIDER: PXD046531 | Pride | 2024-05-23

REPOSITORIES: Pride

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			Action	DRS
	B09256_Bp_WEN12_trap5_PRC-5419_EwaKalemba_shotgun_22.raw	Raw
	B09258_Bp_WEN12_trap5_PRC-5419_EwaKalemba_shotgun_23.raw	Raw
	B09260_Bp_WEN12_trap5_PRC-5419_EwaKalemba_shotgun_24.raw	Raw
	B09262_Bp_WEN12_trap5_PRC-5419_EwaKalemba_shotgun_25.raw	Raw
	B09264_Bp_WEN12_trap5_PRC-5419_EwaKalemba_shotgun_26.raw	Raw

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The association of protein-bound methionine sulfoxide with proteomic basis for aging in beech seeds.

Kalemba Ewa Marzena EM Gevaert Kris K Impens Francis F Dufour Sara S Czerwoniec Anna A

BMC plant biology 20240508 1

<h4>Background</h4>European beech (Fagus sylvatica L.) trees produce seeds irregularly; therefore, it is necessary to store beech seeds for forestation. Despite the acquisition of desiccation tolerance during development, beech seeds are classified as intermediate because they lose viability during long-term storage faster than typical orthodox seeds. In this study, beech seeds stored for short (3 years) or long (20 years) periods under optimal conditions and displaying 92 and 30% germination ca ...[more]

PMID: 38714916

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