Dataset Information

SART3 is a Novel Rme2s Reader studied by tandem MS

ABSTRACT: Arginine methylation is a ubiquitous post-translational modification involved in many biological processes such as transcription, cell signaling and RNA splicing. Tudor domains by far are the major effector domain family for arginine methylation mark recognition. Here we characterize SART3 as a novel selective reader for symmetric dimethylarginine (Rme2s) marks. SART3 harbors a series of HAT (Half-a-TPR) repeats that are aromatic-rich, and it is this region that binds Rme2s motifs. An analysis of the reported structure of the HAT repeats of SART3 identified a putative aromatic cage that potentially “read” the Rme2s-modified motifs, and the key aromatic residues required for Rme2s recognition were identified. We further confirm that this Rme2s reader ability is important for SART3-mediated RNA splicing. Together, our studies revealed that the evolutionarily conserved SART3 HAT domain was a novel reader of Rme2s mark involved in RNA splicing.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: William Russell

LAB HEAD: Mark Bedford

PROVIDER: PXD046708 | Pride | 2024-10-17

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	GFP-EV_Bedford-Yalong_2uL_sceHCD_90m-ot120ot15_F_lkp_01Dec22_011.mgf	Mgf
	GFP-EV_Bedford-Yalong_2uL_sceHCD_90m-ot120ot15_F_lkp_01Dec22_011.msf	Msf
	GFP-EV_Bedford-Yalong_2uL_sceHCD_90m-ot120ot15_F_lkp_01Dec22_011.mzid.gz	Mzid
	GFP-EV_Bedford-Yalong_2uL_sceHCD_90m-ot120ot15_F_lkp_01Dec22_011.mzid_GFP-EV_Bedford-Yalong_2uL_sceHCD_90m-ot120ot15_F_lkp_01Dec22_011.MGF	Mzid
	GFP-EV_Bedford-Yalong_2uL_sceHCD_90m-ot120ot15_F_lkp_01Dec22_011.raw	Raw

Items per page:

1 - 5 of 21

Publications

SART3 reads methylarginine-marked glycine- and arginine-rich motifs.

Wang Yalong Y Zhou Jujun J He Wei W Fu Rongjie R Shi Leilei L Dang Ngoc Khoi NK Liu Bin B Xu Han H Cheng Xiaodong X Bedford Mark T MT

Cell reports 20240709 7

Glycine- and arginine-rich (GAR) motifs, commonly found in RNA-binding and -processing proteins, can be symmetrically (SDMA) or asymmetrically (ADMA) dimethylated at the arginine residue by protein arginine methyltransferases. Arginine-methylated protein motifs are usually read by Tudor domain-containing proteins. Here, using a GFP-Trap, we identify a non-Tudor domain protein, squamous cell carcinoma antigen recognized by T cells 3 (SART3), as a reader for SDMA-marked GAR motifs. Structural anal ...[more]

PMID: 38985674

Similar Datasets

Project description:Environmental enrichment (EE) conditions have profound beneficial effects for reinstating cognitive ability in neuropathological disorders like Alzheimerâs disease (AD). While EE benefits involve epigenetic gene control mechanisms that comprise histone acetylation, the histone acetyltransferases (HATs) involved remain largely unknown. Here, we examine a role for Tip60 HAT action in mediating activity- dependent beneficial neuroadaptations to EE using the Drosophila CNS mushroom body (MB) as a well-characterized cognition model. We show that flies raised under EE conditions display enhanced MB axonal outgrowth, synapse protein production, histone acetylation induction and transcriptional activation of cognition linked genes when compared to their genotypically identical siblings raised under isolated conditions. Further, these beneficial changes are impaired in both Tip60 HAT mutant flies and APP neurodegenerative flies. While EE conditions provide only slight beneficial neuroadaptive changes in the APP neurodegenerative fly MB, such positive changes are significantly enhanced by increasing MB Tip60 HAT levels. Our results implicate Tip60 as a critical mediator of EE-induced benefits, and provide insight into synergistic behavioral and epigenetic based approaches for treatment of cognitive disorders. EE has been shown to positively impact gene expression profiles in the mouse brain that are enriched in functions such as neuronal structure, synaptic plasticity and neurotransmission. Thus, we asked whether the EE induced beneficial MB structural and synaptic changes we observe are accompanied by neuroadaptive transcriptional benefits in the Drosophila MB, and if so, is Tip60 HAT action required for this process. To address this question, we accessed EE induced beneficial transcriptional changes using microarray. We crossed our UAS-mCD8-GFP;Tip60E431Q flies or control UAS-mCD8-GFP flies to MB GAL4 OK-107 to simultaneously induce Tip60 HAT loss in the MB while tagging MB cells with GFP. Adult progeny were exposed to EE or ISO conditions. After conditioning, the GFP tagged MB Kenyon neurons were FACs purified from conditioned fly brains from each genotype to enrich for detection of an EE induced MB transcriptional response. RNA was isolated from the purified Kenyon MB neurons and transcriptional changes for each genotype were assessed using microarray analysis