Proteomics

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Identification of the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity in the rat liver.

ABSTRACT: To identify the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity we purified subcellular organelles including cytosol, late endosomes (LE/MVBs) and lysosomes (CMA+) from the rat liver and performed co-Ip experiment with antibodies directed against the major cellular chaperones mediating CMA and eMI. As such, we analyzed Hsc70-interacting proteins in LE/MVB, CMA+ lysosomes and cytosol by performing pull-down experiments (co-IP) using anti-Hsc70 antibodies. A comparative analysis of the Hsc-70 interactomes revealed that proteins bound to Hsc70 in cytosol and LE/MVBs or in cytosol and CMA+ lysosomes were considered eMI or CMA substrates, respectively.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Liver

SUBMITTER: Cristina Clement  

LAB HEAD: Anna-Maria Cuervo

PROVIDER: PXD046797 | Pride | 2024-01-26

REPOSITORIES: Pride

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Action DRS
CC_Sample_1.mgf Mgf
CC_Sample_1.mzid.gz Mzid
CC_Sample_1.raw Raw
CC_Sample_10.mgf Mgf
CC_Sample_10.raw Raw
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Publications

Chaperone-mediated autophagy (CMA) and endosomal microautophagy (eMI) are pathways for selective degradation of cytosolic proteins in lysosomes and late endosomes, respectively. These autophagic processes share as a first step the recognition of the same five-amino-acid motif in substrate proteins by the Hsc70 chaperone, raising the possibility of coordinated activity of both pathways. In this work, we show the existence of a compensatory relationship between CMA and eMI and identify a role for  ...[more]

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