Proteomics

Dataset Information

0

Trinucleotide cap analogs with N6-adenosine modifications: interaction partners in HEK293F cell line


ABSTRACT: A series of trinucleotide cap analogs functionalized with an amine-terminated linker at the guanosine ribose and immobilized on BrCN-activated Sepharose was prepared. These affinity resins AR-1 (Am), AR-2 (m6Am), and AR-3 (Bn6Am) were incubated with HEK293F cell extract in the presence of GTP to limit non-specific interactions. The pulled-down proteins were eluted with the corresponding trinucleotide cap analog (m7GpppAmpG for AR-1, m7Gpppm6AmpG for AR-2, and m7GpppBn6AmpG for AR-3), digested with trypsin, labeled with isobaric tags (TMT), and analyzed by shotgun proteomics. This experiment was performed in order to assess binding preferences of proteins to the resins AR-1, AR-2, and AR-3.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293f Cell

SUBMITTER: Remigiusz Serwa  

LAB HEAD: Jacek Jemielity

PROVIDER: PXD046838 | Pride | 2024-03-08

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
JJ_H.raw Raw
JJ_J.raw Raw
checksum.txt Txt
txt.zip Other
Items per page:
1 - 4 of 4
altmetric image

Publications

Trinucleotide mRNA Cap Analogue <i>N</i>6-Benzylated at the Site of Posttranscriptional <sup>m6</sup>A<sub>m</sub> Mark Facilitates mRNA Purification and Confers Superior Translational Properties In Vitro and In Vivo.

Warminski Marcin M   Trepkowska Edyta E   Smietanski Miroslaw M   Sikorski Pawel J PJ   Baranowski Marek R MR   Bednarczyk Marcelina M   Kedzierska Hanna H   Majewski Bartosz B   Mamot Adam A   Papiernik Diana D   Popielec Agnieszka A   Serwa Remigiusz A RA   Shimanski Brittany A BA   Sklepkiewicz Piotr P   Sklucka Marta M   Sokolowska Olga O   Spiewla Tomasz T   Toczydlowska-Socha Diana D   Warminska Zofia Z   Wolosewicz Karol K   Zuberek Joanna J   Mugridge Jeffrey S JS   Nowis Dominika D   Golab Jakub J   Jemielity Jacek J   Kowalska Joanna J  

Journal of the American Chemical Society 20240305 12


Eukaryotic mRNAs undergo cotranscriptional 5'-end modification with a 7-methylguanosine cap. In higher eukaryotes, the cap carries additional methylations, such as <sup>m6</sup>A<sub>m</sub>─a common epitranscriptomic mark unique to the mRNA 5'-end. This modification is regulated by the Pcif1 methyltransferase and the FTO demethylase, but its biological function is still unknown. Here, we designed and synthesized a trinucleotide FTO-resistant <i>N</i>6-benzyl analogue of the <sup>m6</sup>A<sub>m  ...[more]

Similar Datasets

2024-10-02 | PXD053198 | Pride
2015-12-15 | E-GEOD-76022 | biostudies-arrayexpress
2016-09-27 | PXD003208 | Pride
2016-07-20 | PXD002861 | Pride
2023-03-11 | PXD035038 | Pride
2019-02-18 | GSE84824 | GEO
2014-09-10 | E-GEOD-58478 | biostudies-arrayexpress
2015-06-10 | E-GEOD-69712 | biostudies-arrayexpress
2021-03-09 | E-MTAB-9616 | biostudies-arrayexpress
2014-06-02 | E-GEOD-54991 | biostudies-arrayexpress