Proteomics

Dataset Information

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CoIP-MS based method to identify FNIP1 binding proteins in exercising muscle


ABSTRACT: Using muscle-specific FNIP1 (WT) and FNIP1 (S220A Tg) muscle tissues, we identified approximately 600 potential FNIP1 (WT) or FNIP1 (S220A) binding proteins in exercising muscle tissue of the corresponding mice by the CoIP-MS method, proteomic analysis showed that both FNIP1 (WT) and FNIP1 (S220A) interacted with major mitochondrial proteins, such as the respiratory complex subunits. Notably, FNIP1 (S220A) specifically interacted with additional mitochondrial subunits that were not observed for FNIP1 (WT), including protein involved in NADH metabolism and sulfur cluster binding. These data suggested that non-phosphorylated FNIP1 (S220A) could enhance its interaction with mitochondria.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Muscle

SUBMITTER: Lei Fang  

LAB HEAD: Lei Fang

PROVIDER: PXD046977 | Pride | 2023-11-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20180529_07-08_XLW_TG-E_ProteinSummary.txt Txt
20180529_07.wiff Wiff
20180529_07.wiff.scan Wiff
20180529_08.wiff Wiff
20180529_08.wiff.scan Wiff
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